J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
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J Med Microbiol 54 (2005), 1065-1070; DOI: 10.1099/jmm.0.46194-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Metallo-ß-lactamase IMP-1 in Providencia rettgeri from two different hospitals in Japan

Katsuaki Shiroto1, Yoshikazu Ishii1, Soichiro Kimura1, Jimena Alba1, Kiwao Watanabe2, Yoshiko Matsushima3 and Keizo Yamaguchi1

1Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, 143-8540 Tokyo, Japan 2Institute of Tropical Medicine, Nagasaki University, Japan 3Department of Clinical Laboratory, Mie University Hospital, Japan

Correspondence Yoshikazu Ishii yoishii{at}med.toho-u.ac.jp

Received June 7, 2005
Accepted July 23, 2005

In 2002, 495 indole-positive proteae strains were isolated from patients at 60 hospitals in Japan. Nine indole-positive proteae strains had reduced susceptibility to imipenem (MIC >= 8 µg ml–1) and were identified as Providencia rettgeri by BD Phoenix. Eight of the nine Prov. rettgeri isolates were confirmed as metallo-ß-lactamase producers by the double-disc synergy test. All the metallo-ß-lactamases were classified as IMP-1 by PCR and DNA sequence analysis. These blaIMP–1 genes were encoded in the integron structure on conjugative plasmids. These plasmids could transfer from Prov. rettgeri clinical isolates to Escherichia coli ML4903 at a frequency between 1.5 x 10–5 and 5.5 x 10–7. The eight blaIMP-positive strains were isolated from two hospitals, and showed two different PFGE patterns, two different integron structures and two different incompatibility groups, which corresponded to the two hospitals. These results strongly suggest the possibility of nosocomial infections by blaIMP–1-producing Prov. rettgeri isolates.

Abbreviations: DDST, double-disc synergy test; ESBL, extended-spectrum ß-lactamase; MßL, metallo-ß-lactamase.




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