HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Saito, R. Articles by Okamura, N. Search for Related Content PubMed PubMed Citation Articles by Saito, R. Articles by Okamura, N. Agricola Articles by Saito, R. Articles by Okamura, N.

Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae

¹Department of Infection Control and Prevention, University of Tokyo Hospital, Bunkyo-ku, Tokyo 113-8655, Japan ²Department of Microbiology and Immunology, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan ³Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, Minato-ku, Tokyo 108-8641, Japan

Correspondence Ryoichi Saito saito-lab{at}h.u-tokyo.ac.jp

Received March 3, 2005
Accepted July 29, 2005

A loop-mediated isothermal amplification (LAMP) assay for the rapid detection of Mycoplasma pneumoniae was developed and evaluated. The assay specifically amplified only M. pneumoniae sequences, and no cross-reactivity was observed for other Mycoplasma species or respiratory bacterial species. The detection limit for this assay was found to be 2 x 10² copies, corresponding to 2–20 colour changing units of M. pneumoniae in 1 h, as observed in a real-time turbidimeter and electrophoretic analysis. The accuracy of the LAMP reaction was confirmed by restriction endonuclease analysis as well as direct sequencing of the amplified product. The assay was applied to 95 nasopharyngeal swab samples collected from patients or from healthy individuals, and compared to a real-time PCR assay in-house. A concordance of 100 % was observed between the two assays. The LAMP assay is easy to perform, shows a rapid reaction and is inexpensive. It may therefore be applied in the routine diagnosis of M. pneumoniae infection in the clinical laboratory.

This article has been cited by other articles:

				J. M. Winchell, K. A. Thurman, S. L. Mitchell, W. L. Thacker, and B. S. Fields Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation J. Clin. Microbiol., September 1, 2008; 46(9): 3116 - 3118. [Abstract] [Full Text] [PDF]

				J. Inacio, O. Flores, and I. Spencer-Martins Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes J. Clin. Microbiol., February 1, 2008; 46(2): 713 - 720. [Abstract] [Full Text] [PDF]

				J.-I. Sekiguchi, T. Asagi, T. Miyoshi-Akiyama, A. Kasai, Y. Mizuguchi, M. Araake, T. Fujino, H. Kikuchi, S. Sasaki, H. Watari, et al. Outbreaks of Multidrug-Resistant Pseudomonas aeruginosa in Community Hospitals in Japan J. Clin. Microbiol., March 1, 2007; 45(3): 979 - 989. [Abstract] [Full Text] [PDF]