HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Grahn, N. Articles by Monstein, H.-J. Search for Related Content PubMed PubMed Citation Articles by Grahn, N. Articles by Monstein, H.-J. Agricola Articles by Grahn, N. Articles by Monstein, H.-J.

Molecular identification of Helicobacter DNA present in human colorectal adenocarcinomas by 16S rDNA PCR amplification and pyrosequencing analysis

¹Molecular Biology Laboratory - LMC, University Hospital, S-581 85 Linköping, Sweden ²Division of Cell Biology, Department of Biomedicine and Surgery, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden ³Laboratory of Human Molecular Genetics, Faculty of Medicine of Sfax, Tunisia

Correspondence Hans-Jürg Monstein hanmo{at}ibk.liu.se

Received April 18, 2005
Accepted July 11, 2005

Seroepidemiological studies have indicated that Helicobacter pylori infection might be a possible risk factor for colorectal adenocarcinoma (CRC) development. However, limited information is available as to whether or not Helicobacter species are present in CRC tissues. In this study the presence of Helicobacter DNA in 77 CRC biopsies was investigated by means of a Helicobacter species-specific 16S rDNA PCR assay and real-time DNA pyrosequencing of the 16S rDNA variable V3 region. Pyrosequencing revealed the presence of Helicobacter DNA sequences in 21 of 77 biopsy specimens (27 %). 16S rDNA sequences corresponding to H. pylori 26695 and H. pylori J99 were most commonly found. Intriguingly, one sequence belonged to Helicobacter mustelae, previously identified in ferrets. No significant correlations were found in the prevalence of Helicobacter DNA between colon and rectum tumour biopsies (P = 0.815), nor between Dukes’ classes A/B and C/D (P = 0.262). 16S rDNA PCR amplification combined with pyrosequencing analysis of 16S rDNA variable V3 regions provides a powerful molecular tool to identify Helicobacter species in human biopsy specimens.

This article has been cited by other articles:

				A. N. Burnett-Hartman, P. A. Newcomb, and J. D. Potter Infectious Agents and Colorectal Cancer: A Review of Helicobacter pylori, Streptococcus bovis, JC Virus, and Human Papillomavirus Cancer Epidemiol. Biomarkers Prev., November 1, 2008; 17(11): 2970 - 2979. [Abstract] [Full Text] [PDF]

				M. Bulajic, B. Stimec, R. Jesenofsky, D. Kecmanovic, M. Ceranic, N. Kostic, W. Schneider-Brachert, A. Lowenfels, P. Maisonneuve, and J.-M. Lohr Helicobacter pylori in Colorectal Carcinoma Tissue Cancer Epidemiol. Biomarkers Prev., March 1, 2007; 16(3): 631 - 633. [Full Text] [PDF]