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J Med Microbiol 54 (2005), 955-957; DOI: 10.1099/jmm.0.45997-0
© 2005 Society for General Microbiology
ISSN 0022-2615

In vitro testing of fungicidal activity of biocides against Aspergillus fumigatus

Anna Maria Tortorano1, Maria Anna Viviani1, Emanuela Biraghi1, Anna Lisa Rigoni1, Anna Prigitano1, Reée Grillot2 and the EBGA Network

1Istituto di Igiene e Medicina Preventiva, Università degli Studi di Milano – IRCCS Ospedale Maggiore, via F. Sforza 35, 20122 Milano, Italy 2Laboratoire ‘Interactions Cellulaires Parasites-Hôte', Université J. Fourier, Grenoble, France

Correspondence Anna Maria Tortorano annamaria.tortorano{at}unimi.it

Received December 24, 2004
Accepted June 27, 2005

The activity of biocides against Aspergillus fumigatus is unknown. In the European guidelines to evaluate the fungicidal activity of a biocide, the critical step concerning the preparation of conidial suspensions is cumbersome and time-consuming. The aims of this study were to evaluate a simplified procedure to prepare conidial suspensions to test a biocide in comparison with the recommended one and to investigate the in vitro activity of seven biocides by the suspensio–neutralization method against A. fumigatus clinical isolates. The proposed simplified procedure proved reproducible, gave the same results and was quicker than that described in the European guidelines. Benzalkonium chloride (0.25 %), glutaraldehyde (1.6 %), polyvinylpyrrolidone iodine (1 % available iodine) and polyester glycol iodine (0.18 % available iodine) showed biocidal activity in <=5 min contact time, and chlorine (0.14 % available Cl) and chlorhexidine (0.06 %) after 15 min and 30–60 min, respectively. In contrast, chloramine-T (0.01 %) did not show biocidal activity. In addition, a simplified and reproducible procedure may be used for testing the fungicidal activity of new compounds or combined formulations. In conclusion, the biocides tested, which are commonly used in hospital settings, were shown to display biocidal activity against A. fumigatus and a simplified procedure may be adopted for testing the fungicidal activity of new compounds.

{dagger}Members of the EBGA Network are listed in the Acknowledgements.




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