J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pourshafie, M
Right arrow Articles by Popoff, M
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pourshafie, M
Right arrow Articles by Popoff, M
Agricola
Right arrow Articles by Pourshafie, M
Right arrow Articles by Popoff, M
J Med Microbiol 54 (2005), 933-936; DOI: 10.1099/jmm.0.46139-0
© 2005 Society for General Microbiology
ISSN 0022-2615

Genotyping Clostridium botulinum toxinotype A isolates from patients using amplified rDNA restriction analysis

M Pourshafie1, P Vahdani2 and M Popoff3

1Pasteur Institute of Iran, Department of Bacteriology, Pasteur Avenue, Tehran, Iran 2Logman Hospital, Department of Infectious Diseases, Tehran, Iran 3Institut Pasteur, Unité des Anaérobies, 25–28 Rue du Dr Roux, F-75724, Paris Cedex 15, France

Correspondence M. Pourshafie pour{at}pasteur.ac.ir

Received April 27, 2005
Accepted June 27, 2005

In this study, the application of amplified rDNA restriction analysis (ARDRA) for characterizing Clostridium botulinum toxinotype A strains isolated from individuals with botulism was evaluated. Ten restriction enzymes were tested for their suitability in ARDRA as a typing method and HhaI was selected for the best outcome. Analysis of HhaI restriction profiles of the amplified products divided C. botulinum isolates into three clusters. Non-toxigenic Clostridium sporogenes strains showed an ARDRA restriction pattern that was distinct from those observed for C. botulinum. The successful use of ARDRA for subdivision of C. botulinum in this study confirmed that this technique is a powerful method for typing of C. botulinum toxinotype A clonal diversity. In addition, it is rapid, sensitive and simple.

Abbreviations: ARDRA, amplified rDNA restriction analysis; RAPD, randomly amplified polymorphic DNA.




This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
T. E. Macdonald, C. H. Helma, L. O. Ticknor, P. J. Jackson, R. T. Okinaka, L. A. Smith, T. J. Smith, and K. K. Hill
Differentiation of Clostridium botulinum Serotype A Strains by Multiple-Locus Variable-Number Tandem-Repeat Analysis
Appl. Envir. Microbiol., February 1, 2008; 74(3): 875 - 882.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2005 Society for General Microbiology.