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J Med Microbiol 53 (2004), 869-874; DOI: 10.1099/jmm.0.45533-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Sensitivity of the BacT/ALERT FA-medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature-dependence

Irene Seegmüller, Ursula Eschenbach, Klaus Kamereck and Thomas Miethke

Institute of Medical Microbiology, Immunology and Hygiene, Technical University of Munich, Trogerstrasse 9, 81675 Munich, Germany

Correspondence Thomas Miethke Thomas.Miethke{at}lrz.tu-muenchen.de

Received November 10, 2003
Accepted April 15, 2004

The BacT/ALERT FA-medium was evaluated to detect Pseudomonas aeruginosa in pre-incubated blood samples. As published previously its predecessor the BacT/ALERT FAN-medium failed to detect P. aeruginosa in delayed entry samples. It is now reported that FA-medium tolerates a longer pre-incubation period at 36 °C, i.e. 8 h, before detection of P. aeruginosa fails in experimentally inoculated blood cultures. In clinical blood samples the frequency of false-negative results concerning P. aeruginosa was reduced from 46.9 % (FAN-medium) to 9.1 % (FA-medium). If media inoculated with P. aeruginosa are pre-incubated at room temperature for 24 h, false-negative results are not observed. Various micro-organisms (Haemophilus influenzae, Streptococcus pneumoniae, Enterobacteriaceae, Staphylococcus aureus, Enterococcus faecalis, Candida glabrata) were recognized after pre-incubation at room temperature with similar sensitivity compared to pre-incubation at 36 °C. It is concluded that FA-medium detects P. aeruginosa in delayed entry samples with increased sensitivity and pre-incubation at room temperature is superior to pre-incubation at 36 °C.




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R. L. Sautter, A. R. Bills, D. L. Lang, G. Ruschell, B. J. Heiter, and P. P. Bourbeau
Effects of Delayed-Entry Conditions on the Recovery and Detection of Microorganisms from BacT/ALERT and BACTEC Blood Culture Bottles
J. Clin. Microbiol., April 1, 2006; 44(4): 1245 - 1249.
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