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J Med Microbiol 53 (2004), 855-860; DOI: 10.1099/jmm.0.45548-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Sensitivity of Helicobacter pylori to an innate defence mechanism, the lactoperoxidase system, in buffer and in human whole saliva

Anna Haukioja1, Riikka Ihalin1, Vuokko Loimaranta2, Marianne Lenander3 and Jorma Tenovuo1

1Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, FIN-20520 Turku, Finland 2Department of Medical Biochemistry, University of Turku, Kiinanmyllynkatu 10, FIN-20520 Turku, Finland 3Kårkulla samkommun, Kårkullavägen 142, FIN-21610 Kirjala, Finland

Correspondence Anna Haukioja anna.haukioja{at}utu.fi

Received November 25, 2003
Accepted April 30, 2004

Helicobacter pylori has frequently been isolated from human dental plaque, and oral spread via saliva is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of H. pylori to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (H2O2) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested H. pylori strains, ATCC 43504T and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of H2O2 in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of H2O2 generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against H. pylori, nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.

Abbreviation: LP, lactoperoxidase.






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