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J Med Microbiol 53 (2004), 783-786; DOI: 10.1099/jmm.0.05425-0
© 2004 Society for General Microbiology
ISSN 0022-2615

A fast, practical and reproducible procedure for the standardization of the cell density of an Aspergillus suspension

Ricardo Araujo1,2, Acacio G. Rodrigues1,2 and Cidalia Pina-Vaz1,2

1Department of Microbiology, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal 2IPATIMUP, University of Porto, Portugal

Correspondence Acacio G. Rodrigues micteam{at}clix.pt

Received August 12, 2003
Accepted February 6, 2004

The progressive increase of invasive disease and reports of resistance among Aspergillus species emphasizes the need for reproducible antifungal susceptibility testing. Inoculum standardization is a crucial step in such procedures. The objective of this study was to develop a fast and precise method of evaluating the cell density of an Aspergillus spore suspension, as an alternative to spectrometric readings or cell-counting with a haemocytometer. Densimat (bioMérieux) is a portable photometer that shows a good correlation with spectrometric readings and can advantageously replace the cumbersome, time-consuming method of cell-counting. Thus, Densimat brings significant improvement to the reproducibility and feasibility of standardization of a fungal inoculum.




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R. Araujo, I. Coutinho, and A. Espinel-Ingroff
Rapid method for testing the susceptibility of Aspergillus fumigatus to amphotericin B, itraconazole, voriconazole and posaconazole by assessment of oxygen consumption
J. Antimicrob. Chemother., December 1, 2008; 62(6): 1277 - 1280.
[Abstract] [Full Text] [PDF]


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