HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carroll, I. M. Articles by Smyth, C. J. Search for Related Content PubMed PubMed Citation Articles by Carroll, I. M. Articles by Smyth, C. J. Agricola Articles by Carroll, I. M. Articles by Smyth, C. J.

Microevolution between paired antral and paired antrum and corpus Helicobacter pylori isolates recovered from individual patients

Ian M. Carroll¹, Niyaz Ahmed², Sarah M. Beesley¹, Aleem A. Khan³, Sheikh Ghousunnissa², Colm A.Ó Moráin⁴, C. M. Habibullah³ and Cyril J. Smyth¹

¹Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College, University of Dublin, Dublin 2, Ireland ²Centre for DNA Fingerprinting and Diagnostics (CDFD), Nacharam, Hyderabad, 50 00076 India ³Owaisi Hospital and Research Centre, Deccan College of Medical Sciences, Kanchanbagh, Santoshnagar, Hyderabad, India ⁴Department of Gastroenterology, The Adelaide and Meath Hospital, Tallaght, Dublin 24, Ireland

Correspondence Cyril J. Smyth csmyth{at}tcd.ie

Received August 20, 2003
Accepted February 12, 2004

Sequence variations located at the signal sequence and mid-region within the vacA gene, the 3'-end of the cagA gene, the indel motifs at the 3'-end of the cag pathogenicity island and the regions upstream of the vacA and ribA genes were determined by PCR in 19 paired antral or antrum and corpus Helicobacter pylori isolates obtained at the same endoscopic session, and three antral pairs taken sequentially. Random amplification of polymorphic DNA (RAPD)-PCR and fluorescent amplified fragment length polymorphism (FAFLP)-PCR fingerprinting were applied to these paired clinical isolates. The FAFLP-PCR profiles generated were phylogenetically analysed. For the 22 paired isolates there were no differences within pairs at five of the genetic loci studied. However, six pairs of isolates (27 %), of which four were antrum and corpus pairs, showed differences in the numbers of repeats located at the 3'-end of the cagA gene. RAPD-PCR fingerprinting showed that 16 (73 %) pairs, nine of which were antrum and corpus pairs, possessed identical profiles, while six (27 %) displayed distinctly different profiles, indicating mixed infections. Three of the six pairs showing differences at the 3'-end of the cagA gene yielded identical RAPD-PCR fingerprints. FAFLP-PCR fingerprinting and phylogenetic analysis revealed that all 16 pairs that displayed identical RAPD-PCR profiles had highly similar, but not identical, fingerprints, demonstrating that these pairs were ancestrally related but had undergone minor genomic alterations. Two antrum and corpus pairs of isolates, within the latter group, were isolates obtained from two siblings from the same family. This analysis demonstrated that each sibling was colonized by ancestrally related strains that exhibited differences in vacA genotype characteristics.

Abbreviation: FAFLP, fluorescent amplified fragment length polymorphism.

This article has been cited by other articles:

				R. H. Argent, J. L. Hale, E. M. El-Omar, and J. C. Atherton Differences in Helicobacter pylori CagA tyrosine phosphorylation motif patterns between western and East Asian strains, and influences on interleukin-8 secretion J. Med. Microbiol., September 1, 2008; 57(9): 1062 - 1067. [Abstract] [Full Text] [PDF]

				R. H. Argent, R. J. Thomas, F. Aviles-Jimenez, D. P. Letley, M. C. Limb, E. M. El-Omar, and J. C. Atherton Toxigenic Helicobacter pylori Infection Precedes Gastric Hypochlorhydria in Cancer Relatives, and H. pylori Virulence Evolves in These Families Clin. Cancer Res., April 1, 2008; 14(7): 2227 - 2235. [Abstract] [Full Text] [PDF]

				N. R. Salama, G. Gonzalez-Valencia, B. Deatherage, F. Aviles-Jimenez, J. C. Atherton, D. Y. Graham, and J. Torres Genetic Analysis of Helicobacter pylori Strain Populations Colonizing the Stomach at Different Times Postinfection J. Bacteriol., May 15, 2007; 189(10): 3834 - 3845. [Abstract] [Full Text] [PDF]

				M. J. Matteo, G. Granados, C. V. Perez, M. Olmos, C. Sanchez, and M. Catalano Helicobacter pylori cag pathogenicity island genotype diversity within the gastric niche of a single host J. Med. Microbiol., May 1, 2007; 56(5): 664 - 669. [Abstract] [Full Text] [PDF]