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J Med Microbiol 53 (2004), 539-543; DOI: 10.1099/jmm.0.05510-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Frequency and polymorphism of sopE in isolates of Salmonella enterica belonging to the ten most prevalent serotypes in England and Wales

Katie L. Hopkins and E. John Threlfall

Antimicrobial Resistance and Molecular Epidemiology Unit, Laboratory of Enteric Pathogens, Specialist and Reference Microbiology Division, Health Protection Agency, 61 Colindale Avenue, London NW9 5HT, UK

Correspondence Katie L. Hopkins Katie.Hopkins{at}HPA.org.uk

Received October 17, 2003
Accepted February 10, 2004

Translocated effector protein, SopE, leads to actin cytoskeletal rearrangements and membrane ruffling. Only a subset of Salmonella enterica serotypes possess sopE, with the majority of sopE-carrying S. enterica serotype Typhimurium associated with epidemics. Using real-time PCR and sequencing, sopE was investigated in the ten most prevalent serotypes of S. enterica in England and Wales in 2001. sopE was identified in S. Typhimurium definitive phage types 29, 44, 49, 204b and 204c, all of which either have been involved in major epidemics or are precursors of epidemic strains. The presence of sopE varied in the remaining nine serotypes, but was more common in the top four (Enteritidis, Virchow, Hadar and Newport). Nucleotide changes were detected throughout sopE and may result in altered specificity for certain signal transduction pathways. Since acquisition of sopE may play a key role in emergence of epidemic strains, detection of sopE could aid identification of those Salmonella strains with the potential for epidemic spread.

Abbreviations: DT, definitive phage type; MDR, multidrug-resistant; PT, phage type.

The GenBank accession numbers for the sopE sequences reported in this study are AY167929 (S. Enteritidis), AY167930 (S. Java), AY167931 (S. Infantis), AY168873 (S. Newport), AY168874 (S. Hadar), AY168876 (S. Virchow V1) and AY168875 (S. Virchow V2).

A sopE sequence alignment is available as supplementary material in JMM Online.






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