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J Med Microbiol 53 (2004), 341-343; DOI: 10.1099/jmm.0.05402-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Demonstration of Brachyspira aalborgi lineages 2 and 3 in human colonic biopsies with intestinal spirochaetosis by specific fluorescent in situ hybridization

Tim K. Jensen1, Peter S. Teglbjærg2, Christian F. Lindboe3 and Mette Boye1

1Danish Veterinary Institute, Bülowsvej 27, DK-1790 Copenhagen, Denmark 2Institute of Pathology, Aalborg Hospital, Aalborg, Denmark 3Vest-Agder Sentralsykehus, Kristiansand, Norway

Correspondence Tim K. Jensen tkj{at}DFVF.dk

Received July 31, 2003
Accepted November 25, 2003

Sequences of known 16S rRNA genes, derived from sequence analysis of cloned 16S rDNA, were used to design a specific oligonucleotide probe targeting spirochaetes of Brachyspira aalborgi lineages 2 and 3. The probe was used with fluorescent in situ hybridization to study the involvement of these organisms in human intestinal spirochaetosis. Seventeen human colonic biopsies from Norway and Denmark with intestinal spirochaetosis caused by Brachyspira-like organisms different from the type strain of B. aalborgi (lineage 1) were examined. Application of the probe gave a positive signal in two Norwegian biopsies, whereas the 15 other biopsies were hybridization-negative. The positive reaction visualized the spirochaetes as a fluorescent, 3–5 µm-high fringe on the surface epithelium, extending into the crypts. The study verified the presence of B. aalborgi lineages 2 and 3 and identified the bacteria as an aetiological agent of human intestinal spirochaetosis.

This paper was presented at the Second International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, UK, 2–4 April 2003.

Abbreviation: HIS, human intestinal spirochaetosis.




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