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J Med Microbiol 53 (2004), 301-307; DOI: 10.1099/jmm.0.05500-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Immunomagnetic separation of the intestinal spirochaetes Brachyspira pilosicoli and Brachyspira hyodysenteriae from porcine faeces

Enrique Corona-Barrera1, David G.E. Smith1, Tom La2, David J. Hampson2 and Jill R. Thomson3

1Zoonotic and Animal Pathogens, University of Edinburgh, Edinburgh, Scotland, UK 2School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia, Australia 3Scottish Agricultural College, Veterinary Science Division, Penicuik, Scotland, UK

Correspondence Jill R. Thomson j.thomson{at}ed.sac.ac.uk

Received October 6, 2003
Accepted January 28, 2004

Porcine intestinal spirochaetes are fastidious anaerobic organisms and, as a consequence, it has been necessary to develop various protocols to enhance their isolation from or detection in faeces. Immunomagnetic separation (IMS) is a method developed recently to improve separation of target cells from mixed cell suspensions. The purpose of the present study was to compare the relative sensitivity of IMS for isolation of Brachyspira pilosicoli and Brachyspira hyodysenteriae with current routine diagnostic methods (culture on selective media and PCR) for detection of these micro-organisms in pig faeces. Neither direct nor indirect IMS methods enhanced the sensitivity of detection of either organism when performed with the recommended washings during sample processing. Performance of the IMS procedure without washing gave sensitivity at levels similar to direct culture onto selective medium. Further development of IMS techniques is required to improve isolation rates of Brachyspira species from faecal samples.

This paper was presented at the Second International Conference on Colonic Spirochaetal Infections in Animals and Humans, Edinburgh, UK, 2–4 April 2003.

Abbreviations: IMS, immunomagnetic separation; PCS, porcine colonic spirochaetosis; SD, swine dysentery.




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