HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jonasson, R. Articles by Jensen-Waern, M. Search for Related Content PubMed PubMed Citation Articles by Jonasson, R. Articles by Jensen-Waern, M. Agricola Articles by Jonasson, R. Articles by Jensen-Waern, M.

Differences in lymphocyte subpopulations and cell counts before and after experimentally induced swine dysentery

¹Department of Large Animal Clinical Sciences, Unit of Comparative Physiology and Medicine, Faculty of Veterinary Medicine, Swedish University of Agricultural Sciences, PO Box 7018, S-750 07 Uppsala, Sweden ²Department of Anatomy and Physiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

Correspondence Robert Jonasson Robert.Jonasson{at}kirmed.slu.se

Received June 23, 2003
Accepted October 28, 2003

The aim of this study was to examine the levels of circulating leukocytes and lymphocyte subpopulations before and immediately after experimentally induced swine dysentery. Twenty-one healthy crossbred pigs (22 kg) were orally inoculated with Brachyspira hyodysenteriae. Blood was sampled before inoculation and when clinical signs of swine dysentery occurred. Pigs that remained healthy were sampled when killed. Total and differential white blood cell counts were performed, and lymphocyte subpopulations were analysed using flow cytometry. Following a mean incubation period of 13 days, 12 pigs developed swine dysentery, whereas nine remained healthy throughout the study. Before inoculation, pigs that subsequently developed swine dysentery displayed higher levels of circulating T cells (mean ± SE; 30.7 ± 3.5 %) compared with pigs that remained healthy (14.9 ± 1.4 %). Sick animals also displayed lower levels of CD8⁺ cells (24.6 ± 1.5 %), cytotoxic/suppressor T cells (10.9 ± 1.3 %) and CD4⁺ CD8^- T cells (8.1 ± 1.0 %) than the pigs that remained healthy (34.9 ± 3.1 %; 17.6 ± 2.0 %; 13.6 ± 2.3 %). No difference was observed in leukocyte counts before inoculation. At onset of swine dysentery, there was an increase in monocytes (from 1.5 ± 0.2 x 10⁹ to 3.8 ± 0.5 x 10⁹ l^-1) and CD4⁺ CD8⁺ T cells (from 5.8 ± 0.9 to 8.9 ± 0.7 %). In conclusion, T cells and CD8⁺ cells may be associated with susceptibility to experimentally induced swine dysentery, whereas monocytes and CD4⁺ CD8⁺ T cells appear to be the major responding leukocytes during the disease.

This article has been cited by other articles:

				J. Holderness, L. Jackiw, E. Kimmel, H. Kerns, M. Radke, J. F. Hedges, C. Petrie, P. McCurley, P. M. Glee, A. Palecanda, et al. Select Plant Tannins Induce IL-2R{alpha} Up-Regulation and Augment Cell Division in {gamma}{delta} T Cells J. Immunol., November 15, 2007; 179(10): 6468 - 6478. [Abstract] [Full Text] [PDF]

				R. Jonasson, M. Andersson, T. Rasback, A. Johannisson, and M. Jensen-Waern Immunological alterations during the clinical and recovery phases of experimental swine dysentery. J. Med. Microbiol., July 1, 2006; 55(Pt 7): 845 - 855. [Abstract] [Full Text] [PDF]