HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhou, J. Articles by He, L. Search for Related Content PubMed PubMed Citation Articles by Zhou, J. Articles by He, L. Agricola Articles by Zhou, J. Articles by He, L.

cagA genotype and variants in Chinese Helicobacter pylori strains and relationship to gastroduodenal diseases

Jianchang Zhou^1,2, , Jianzhong Zhang¹, Caipu Xu² and Lihua He¹

¹National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206 Beijing, P.R. China ²Department of Gastroenterology, Southwest Hospital, Third Military Medical University, 400038 Chongqing, P.R. China

Correspondence Jianzhong Zhang helico{at}public.bta.net.cn

Received June 27, 2003
Accepted November 28, 2003

Previous studies have implicated CagA [encoded by cytotoxin-associated gene A (cagA)] in Helicobacter pylori-associated gastroduodenal pathology and distinct subgenotypes of cagA may circulate in different pathological manifestations of cagA-positive H. pylori infection. To investigate cagA genotype and variants in Chinese H. pylori strains and explore their relationship with gastroduodenal diseases, the cagA status of 82 Chinese H. pylori strains was examined and variation in size of the 3' region of cagA in 71 of these strains was analysed by PCR. cagA was detected in 28 (100 %) of 28 strains from peptic ulcer patients, two (100 %) of two strains from gastric cancer patients, 32 (94.1 %) of 34 strains from chronic gastritis patients and 17 (94.4 %) of 18 strains from healthy volunteers. PCR products of the cagA 3' variable region were obtained from 71 (92.2 %) of 77 Chinese H. pylori strains and could be classified into subgenotypes I, II and III, which gave PCR products of around 825, 900 and 950 bp, respectively. Subgenotype I cagA predominated in Chinese H. pylori strains (67/71), whereas subgenotype II cagA presented in two isolates from patients with chronic gastritis and subgenotype III presented in two isolates from healthy volunteers. Therefore, neither cagA nor its 3' region variants can be used as a sole marker for the presence of particular H. pylori-related gastroduodenal diseases in the Chinese population.

Abbreviation: cagA, cytotoxin-associated gene A.

This article has been cited by other articles:

				R. J. Owen and J. Xerry Geographical conservation of short inserts in the signal and middle regions of the Helicobacter pylori vacuolating cytotoxin gene Microbiology, April 1, 2007; 153(4): 1176 - 1186. [Abstract] [Full Text] [PDF]

				Y. Zhang, R. H. Argent, D. P. Letley, R. J. Thomas, and J. C. Atherton Tyrosine Phosphorylation of CagA from Chinese Helicobacter pylori Isolates in AGS Gastric Epithelial Cells J. Clin. Microbiol., February 1, 2005; 43(2): 786 - 790. [Abstract] [Full Text] [PDF]