| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1National Institute of Public Health, 
robárova 48, 100 42 Prague, Czech Republic 2Department of Infectious Diseases, Leiden University Medical Center C5-P, PO Box 9600, 2300 RC Leiden, the Netherlands
Correspondence Alexandr Nemec anemec{at}szu.cz
Received August 24, 2003
Accepted November 11, 2003
In a recent study, a large proportion of multi-drug-resistant (MDR) Acinetobacter baumannii strains that were isolated from hospitalized patients in the Czech Republic was found to belong to two major groups (A and B). These groups appeared to be similar to epidemic clones I and II, respectively, which were identified previously among outbreak strains from north-western European hospitals. The aim of the present study was to assess in detail the genetic relatedness of Czech A. baumannii strains and those of epidemic clones I and II by using ribotyping with HindIII and HincII and by AFLP fingerprinting. The study collection included 70 MDR strains that were isolated in 30 Czech hospitals in 1991–2001, 15 susceptible Czech strains from 1991 to 1996 and 13 reference strains of clones I and II from 1982 to 1990. One major HindIII/HincIII ribotype (R1-1) was observed in 38 MDR Czech strains and eight reference strains of clone I, whereas another major ribotype (R2-2) was observed in 11 MDR Czech strains and in three reference strains of clone II. A selection of 59 Czech strains (representative of all ribotypes) and the 13 reference strains were investigated by AFLP fingerprinting. At a clustering level of 83 %, two large clusters could be distinguished: cluster 1 included all reference strains of clone I and 25 MDR Czech strains, whilst cluster 2 contained all reference strains of clone II and 11 MDR Czech strains. There was a clear correlation between the groupings by AFLP analysis and by ribotyping, as all strains with ribotype R1-1 and four strains with slightly different ribotypes were found in AFLP cluster 1, whereas all strains with ribotype R2-2 and seven strains with similar ribotypes were in AFLP cluster 2. Thus, 41 and 21 MDR Czech strains could be classified as belonging to clones I and II, respectively. The remaining eight MDR and 15 susceptible strains were highly heterogeneous and were distinct from clones I and II by both AFLP fingerprinting and ribotyping. These results indicate that the two predominant groups observed among MDR Czech A. baumannii strains from the 1990s are genetically congruent with the north-western European epidemic clones that were found in the 1980s. Recognition of these clinically relevant, widespread clones is important in infection prevention and control; they are also interesting subjects to study genetic mechanisms that give rise to their antibiotic resistance and epidemic behaviour.
A table showing data on origin and properties of the strains used in this study is available in JMM Online.
This article has been cited by other articles:
|
|
 |
|
  A. Y. Peleg, H. Seifert, and D. L. Paterson Acinetobacter baumannii: Emergence of a Successful Pathogen Clin. Microbiol. Rev., July 1, 2008; 21(3): 538 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Iacono, L. Villa, D. Fortini, R. Bordoni, F. Imperi, R. J. P. Bonnal, T. Sicheritz-Ponten, G. De Bellis, P. Visca, A. Cassone, et al. Whole-Genome Pyrosequencing of an Epidemic Multidrug-Resistant Acinetobacter baumannii Strain Belonging to the European Clone II Group Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2616 - 2625. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Nemec, L. Krizova, M. Maixnerova, L. Diancourt, T. J. K. van der Reijden, S. Brisse, P. van den Broek, and L. Dijkshoorn Emergence of carbapenem resistance in Acinetobacter baumannii in the Czech Republic is associated with the spread of multidrug-resistant strains of European clone II J. Antimicrob. Chemother., May 13, 2008; (2008) dkn205v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. W. Loehfelm, N. R. Luke, and A. A. Campagnari Identification and Characterization of an Acinetobacter baumannii Biofilm-Associated Protein J. Bacteriol., February 1, 2008; 190(3): 1036 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nemec, M. Maixnerova, T. J. K. van der Reijden, P. J. van den Broek, and L. Dijkshoorn Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains J. Antimicrob. Chemother., September 1, 2007; 60(3): 483 - 489. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Scheetz, C. Qi, J. R. Warren, M. J. Postelnick, T. Zembower, A. Obias, and G. A. Noskin In Vitro Activities of Various Antimicrobials Alone and in Combination with Tigecycline against Carbapenem-Intermediate or -Resistant Acinetobacter baumannii Antimicrob. Agents Chemother., May 1, 2007; 51(5): 1621 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Valenzuela, L. Thomas, S. R. Partridge, T. van der Reijden, L. Dijkshoorn, and J. Iredell Horizontal Gene Transfer in a Polyclonal Outbreak of Carbapenem-Resistant Acinetobacter baumannii J. Clin. Microbiol., February 1, 2007; 45(2): 453 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Huys, M. Cnockaert, A. Nemec, and J. Swings Sequence-Based Typing of adeB as a Potential Tool To Identify Intraspecific Groups among Clinical Strains of Multidrug-Resistant Acinetobacter baumannii J. Clin. Microbiol., October 1, 2005; 43(10): 5327 - 5331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Seifert, L. Dolzani, R. Bressan, T. van der Reijden, B. van Strijen, D. Stefanik, H. Heersma, and L. Dijkshoorn Standardization and Interlaboratory Reproducibility Assessment of Pulsed-Field Gel Electrophoresis-Generated Fingerprints of Acinetobacter baumannii J. Clin. Microbiol., September 1, 2005; 43(9): 4328 - 4335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Bartual, H. Seifert, C. Hippler, M. A. D. Luzon, H. Wisplinghoff, and F. Rodriguez-Valera Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii J. Clin. Microbiol., September 1, 2005; 43(9): 4382 - 4390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Huys, M. Cnockaert, A. Nemec, L. Dijkshoorn, S. Brisse, M. Vaneechoutte, and J. Swings Repetitive-DNA-element PCR fingerprinting and antibiotic resistance of pan-European multi-resistant Acinetobacter baumannii clone III strains J. Med. Microbiol., September 1, 2005; 54(9): 851 - 856. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Dijkshoorn, C. P. J. M. Brouwer, S. J. P. Bogaards, A. Nemec, P. J. van den Broek, and P. H. Nibbering The Synthetic N-Terminal Peptide of Human Lactoferrin, hLF(1-11), Is Highly Effective against Experimental Infection Caused by Multidrug-Resistant Acinetobacter baumannii Antimicrob. Agents Chemother., December 1, 2004; 48(12): 4919 - 4921. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Nemec, L. Dolzani, S. Brisse, P. van den Broek, and L. Dijkshoorn Diversity of aminoglycoside-resistance genes and their association with class 1 integrons among strains of pan-European Acinetobacter baumannii clones J. Med. Microbiol., December 1, 2004; 53(12): 1233 - 1240. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | J MED MICROBIOL | MICROBIOLOGY | J GEN VIROL | ALL SGM JOURNALS |
