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J Med Microbiol 53 (2004), 119-123; DOI: 10.1099/jmm.0.05436-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Comparison of ITS and IGS1 regions for strain typing of clinical and non-clinical isolates of Pichia anomala

Rajeshwari Sutar1, Joseph K. David2, K. Ganesan1, Anup K. Ghosh2{dagger}, Sunit Singhi2, Arunaloke Chakrabarti2 and Anand K. Bachhawat1

1Institute of Microbial Technology, Sector 39-A, Chandigarh, India 160 036 2Postgraduate Institute of Medical Education and Research, Chandigarh, India 160 012

Correspondence Anand K. Bachhawat akbachhawat{at}hotmail.com

Received August 16, 2003
Accepted November 17, 2003

Pichia anomala is an emerging nosocomial pathogen and there is a need for methods that distinguish between different P. anomala strains. In the typing of several clinical as well as non-clinical P. anomala strains, the sequence variation of the internal transcribed spacer (ITS) was found to be inadequate for typing purposes. The intergenic spacer 1 (IGS1) region of the rDNA of several P. anomala strains was therefore investigated in detail. The IGS1 region (which varied from 1213 to 1231 bp in length) was interspersed with repeats and had more variation than the ITS regions. Comparative analysis in cases where analysis by the ITS was ambiguous clearly revealed the IGS1 region to be a more discriminatory tool in the typing of P. anomala strains.

{dagger}Present address: Georgetown University, Microbiology and Immunology, 3rd Floor, Med-Dental Building, 3900 Reservoir Road, Washington, DC 20007, USA.

Abbreviations: IGS, intergenic spacer; ITS, internal transcribed spacer.

The GenBank/EMBL/DDBJ accession numbers for the IGS1 and ITS sequences of P. anomala strains determined in this study are AY231599–AY231612, as detailed in Table 1.




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S. Bhardwaj, R. Sutar, A. K. Bachhawat, S. Singhi, and A. Chakrabarti
PCR-based identification and strain typing of Pichia anomala using the ribosomal intergenic spacer region IGS1
J. Med. Microbiol., February 1, 2007; 56(2): 185 - 189.
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