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J Med Microbiol 53 (2004), 115-117; DOI: 10.1099/jmm.0.05416-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Automation of a fluorescence-based multiplex PCR for the laboratory confirmation of common bacterial pathogens

Karen Smith1, Mathew A. Diggle1 and Stuart C. Clarke1,2

1Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital, Glasgow, Scotland, UK 2Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Scotland, UK

Correspondence Stuart C. Clarke stuart.clarke{at}northglasgow.scot.nhs.uk

Received August 7, 2003
Accepted October 22, 2003

A fluorescence-based multiplex PCR was automated for the simultaneous detection of Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae in clinical samples from patients with suspected meningitis. Sensitivity of one to two genome copies per 100 µl sample and specificity of 100 % for each organism were shown. Automation of DNA extraction, liquid handling, PCR and analysis are achieved on a single platform, which enables a high throughput and rapid turnaround of clinical samples that, in turn, leads to faster diagnosis. This is ultimately beneficial to the treatment of the patient and for public health management.

Abbreviations: CSF, cerebrospinal fluid; SMPRL, Scottish Meningococcus and Pneumococcus Reference Laboratory.




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