HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ramaswamy, S. V. Articles by Graviss, E. A. Search for Related Content PubMed PubMed Citation Articles by Ramaswamy, S. V. Articles by Graviss, E. A. Agricola Articles by Ramaswamy, S. V. Articles by Graviss, E. A.

Genotypic analysis of multidrug-resistant Mycobacterium tuberculosis isolates from Monterrey, Mexico

Srinivas V. Ramaswamy¹, Shu-Jun Dou¹, Adrian Rendon², Zhenhua Yang^3,4, M. Donald Cave^3,5 and Edward A. Graviss¹

¹Houston Tuberculosis Initiative, Department of Pathology, Baylor College of Medicine, Houston, TX, USA ²Pulmonary Services and Clinical Pathology Laboratory, University Hospital of Monterrey, Universidad Autonomy de Nuevo Leon, Nuevo Leon, Mexico ³Regional Tuberculosis Genotyping Laboratory, Central Arkansas Veterans Healthcare System, AR, USA ⁴Department of Medicine and ⁵Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock, AR, USA

Correspondence Edward A. Graviss egraviss{at}bcm.tmc.edu

Received June 10, 2003
Accepted November 13, 2003

Thirty-seven multidrug-resistant and 13 pan-susceptible isolates of Mycobacterium tuberculosis were analysed for the diversity of genotypes associated with known drug-resistance mechanisms. The isolates were obtained from patients attending a university tuberculosis clinic in Monterrey, Mexico. A total of 25 IS6110-RFLP patterns were obtained from the multidrug-resistant tuberculosis (MDR-TB) isolates. Approximately 65 % of the MDR-TB isolates were attributed to secondary resistance. Different drug-susceptibility patterns were seen with the clustered isolates. The percentage of isolates resistant to isoniazid (INH), rifampicin (RIF), ethambutol (EMB) and streptomycin (STR) was 100, 97.3, 48.7 and 67.6, respectively. The most common resistance-associated polymorphisms for the four drugs were as follows: INH, Ser315Thr (67.6 %) in katG; RIF, Ser450Leu (41.7 %) in rpoB; EMB, Met306Ile/Val/Leu (66.7 %) in embB; and STR, Lys43Arg (24 %) in rpsL. Drug-resistance-associated mutations were similar to changes occurring in isolates from other areas of the world, but unique, previously unreported, mutations in katG (n = 5), rpoB (n = 1) and rrs (n = 3) were also identified.

Abbreviations: EMB, ethambutol; INH, isoniazid; MDR-TB, multidrug-resistant tuberculosis; PZA, pyrazinamide; RIF, rifampicin; STR, streptomycin.

This article has been cited by other articles:

				F. S. Spies, P. E. Almeida da Silva, M. O. Ribeiro, M. L. Rossetti, and A. Zaha Identification of Mutations Related to Streptomycin Resistance in Clinical Isolates of Mycobacterium tuberculosis and Possible Involvement of Efflux Mechanism Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2947 - 2949. [Abstract] [Full Text] [PDF]

				M. Brimacombe, M. Hazbon, A. S. Motiwala, and D. Alland Antibiotic Resistance and Single-Nucleotide Polymorphism Cluster Grouping Type in a Multinational Sample of Resistant Mycobacterium tuberculosis Isolates Antimicrob. Agents Chemother., November 1, 2007; 51(11): 4157 - 4159. [Abstract] [Full Text] [PDF]

				A. Soudani, S. Hadjfredj, M. Zribi, A. Masmoudi, T. Messaoud, H. Tiouri, and C. Fendri Characterization of Tunisian Mycobacterium tuberculosis Rifampin-Resistant Clinical Isolates J. Clin. Microbiol., September 1, 2007; 45(9): 3095 - 3097. [Abstract] [Full Text] [PDF]

				M. H. Hazbon, M. Brimacombe, M. Bobadilla del Valle, M. Cavatore, M. I. Guerrero, M. Varma-Basil, H. Billman-Jacobe, C. Lavender, J. Fyfe, L. Garcia-Garcia, et al. Population Genetics Study of Isoniazid Resistance Mutations and Evolution of Multidrug-Resistant Mycobacterium tuberculosis. Antimicrob. Agents Chemother., August 1, 2006; 50(8): 2640 - 2649. [Abstract] [Full Text] [PDF]

				L. J. Alderwick, E. Radmacher, M. Seidel, R. Gande, P. G. Hitchen, H. R. Morris, A. Dell, H. Sahm, L. Eggeling, and G. S. Besra Deletion of Cg-emb in Corynebacterianeae Leads to a Novel Truncated Cell Wall Arabinogalactan, whereas Inactivation of Cg-ubiA Results in an Arabinan-deficient Mutant with a Cell Wall Galactan Core J. Biol. Chem., September 16, 2005; 280(37): 32362 - 32371. [Abstract] [Full Text] [PDF]

				D. M. O'Sullivan, T. D. McHugh, and S. H. Gillespie Analysis of rpoB and pncA mutations in the published literature: an insight into the role of oxidative stress in Mycobacterium tuberculosis evolution? J. Antimicrob. Chemother., May 1, 2005; 55(5): 674 - 679. [Abstract] [Full Text] [PDF]

				A. S. G. Lee, S. N. K. Othman, Y. M. Ho, and S. Y. Wong Novel Mutations within the embB Gene in Ethambutol-Susceptible Clinical Isolates of Mycobacterium tuberculosis Antimicrob. Agents Chemother., November 1, 2004; 48(11): 4447 - 4449. [Abstract] [Full Text] [PDF]