J Med Microbiol International Journal of Systematic and Evolutionary Microbiology
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J Med Microbiol 53 (2004), 1195-1199; DOI: 10.1099/jmm.0.45697-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Evaluation of phenotypic methods for methicillin resistance characterization in coagulase-negative staphylococci (CNS)

Juliana Caierão1, Maiara Musskopf1, Silvana Superti2, Eliane Roesch3, Cícero G Dias1,2 and Pedro A d'Azevedo1

1Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Rua Sarmento Leite 245/211, Porto Alegre, RS 90050-170, Brazil 2Hospital Mãe de Deus, Porto Alegre, Brazil 3Laboratório Central de Análises Clínicas da Irmandade Santa Casa de Misericórdia de Porto Alegre, RS, Brazil

Correspondence Pedro A. d'Azevedo pedroaze{at}fffcmpa.tche.br

Received April 9, 2004
Accepted August 5, 2004

Coagulase-negative staphylococci (CNS) are the major cause of nosocomial infections. Methicillin-resistant strains are particularly important because they narrow therapeutic options. Detecting methicillin resistance among CNS has been a challenge for years. The objective of this study was to determine the accuracy of an agar screening test (0.6 and 4 µg oxacillin ml–1), disc diffusion and the automated MicroScan system to characterize methicillin resistance among CNS. One hundred and seventy five strains were analysed: 41.1 % Staphylococcus epidermidis and 59.9 % other species; 69.1 % were mecA-positive. The results showed that the methods have optimal correlation with the detection of mecA gene for S. epidermidis, Staphylococcus hominis and Staphylococcus haemolyticus. However, accuracy of the tests is impaired when less common species are analysed. The only 100 % accurate test was agar screening with 4 µg oxacillin ml–1.

Abbreviations: CNS, coagulase-negative staphylococci; PBP, penicillin-binding protein.






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