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J Med Microbiol 53 (2004), 1105-1107; DOI: 10.1099/jmm.0.45744-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Assessing clonality of Vibrio cholerae Inaba isolates by characterization of nonsense mutations in wbeT

Sjoerd G Rijpkema1, Zarmina Durrani1, T Ramamurthy2 and G Balakrish Nair3

1Division of Bacteriology, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK 2National Institute of Cholera and Enteric Diseases, Calcutta, India 3Laboratory Sciences Division, ICDDR, B: Centre for Health and Population Research, Mohakhali, Dhaka 1212, Bangladesh

Correspondence Sjoerd G. Rijpkema srijpkema{at}nibsc.ac.uk

Received May 17, 2004
Accepted July 29, 2004

The transferase gene wbeT of six clinical isolates of Vibrio cholerae O1 biotype El Tor was analysed. Two unique mutations were identified in the wbeT gene of three Inaba isolates. Due to their random nature, mutations in wbeT can be used to determine the clonal origin of clinical Inaba isolates.

The GenBank/EMBL/DDBJ accession numbers for the wbeT sequences of V. cholerae determined in this study are AY458621–AY458626.




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