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J Med Microbiol 53 (2004), 1053-1064; DOI: 10.1099/jmm.0.45661-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Role of quorum sensing in the pathogenicity of Burkholderia pseudomallei

Ricky L Ulrich1, David DeShazer1, Ernst E Brueggemann2, Harry B Hines2, Petra C Oyston3 and Jeffrey A Jeddeloh4

1,2Bacteriology Division1 and Toxinology/Aerobiology Division2, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD 21702-5011, USA 3Microbiology, Dstl, CBS Porton Down, Salisbury SP4 0JQ, UK 4Orion Genomics, Center for Emerging Technologies, 4041 Forest Park, St. Louis, MO 63108, USA

Correspondence Ricky L. Ulrich Ricky.Ulrich{at}AMEDD.ARMY.MIL

Received March 4, 2004
Accepted June 25, 2004

Burkholderia pseudomallei is the causative agent of human and animal melioidosis. The role of quorum sensing (QS) in the in vivo pathogenicity of B. pseudomallei via inhalational exposure of BALB/c mice and intraperitoneal challenge of Syrian hamsters has not been reported. This investigation demonstrates that B. pseudomallei encodes a minimum of three luxI and five luxR homologues that are involved in animal pathogenicity. Mass spectrometry analysis of culture supernatants revealed that wild-type B. pseudomallei and the luxI mutants synthesized numerous signalling molecules, including N-octanoyl-homoserine lactone, N-decanoyl-homoserine lactone, N-(3-hydroxyoctanoyl)-L-homoserine lactone, N-(3-hydroxydecanoyl)-L-homoserine lactone and N-(3-oxotetradecanoyl)-L-homoserine lactone, which was further confirmed by heterologous expression of the B. pseudomallei luxI alleles in Escherichia coli. Mutagenesis of the B. pseudomallei QS system increased the time to death and reduced organ colonization of aerosolized BALB/c mice. Further, intraperitoneal challenge of Syrian hamsters with the B. pseudomallei QS mutants resulted in a significant increase in the LD50. Using semi-quantitative plate assays, preliminary analysis suggests that QS does not affect lipase, protease and phospholipase C biosynthesis/secretion in B. pseudomallei. The findings of the investigation demonstrate that B. pseudomallei encodes multiple luxIR genes, and disruption of the QS alleles reduces animal pathogenicity, but does not affect exoproduct secretion.

Abbreviations: AHL, N-acyl-homoserine lactone; C8-HSL, N-octanoyl-homoserine lactone; C10-HSL, N-decanoyl-homoserine lactone; 3-hydroxy-C8-HSL, N-(3-hydroxyoctanoyl)-L-homoserine lactone; 3-hydroxy-C10-HSL, N-(3-hydroxydecanoyl)-L-homoserine lactone; 3-oxo-C14-HSL, N-(3-oxotetradecanoyl)-L-homoserine lactone; QS, quorum sensing.




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