J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Souza, A. V.
Right arrow Articles by Magalhães, V. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Souza, A. V.
Right arrow Articles by Magalhães, V. D.
Agricola
Right arrow Articles by Souza, A. V.
Right arrow Articles by Magalhães, V. D.
J Med Microbiol 53 (2004), 999-1005; DOI: 10.1099/jmm.0.45702-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit

Andrea V. Souza1, Cláudia R. Moreira1, Jacyr Pasternak2,3, Maria de Lurdes Hirata2, Denise Alves Saltini2, Viviane Cristina Caetano2, Suely Ciosak2, Fátima M. Azevedo1, Patricia Severino1, Peter Vandamme4 and Vanda D. Magalhães1

1Centro de Pesquisa Experimental, Instituto de Ensino e Pesquisa Albert Einstein - Av. Albert Einstein 627, 05651-901, São Paulo, SP, Brazil 2Infection Control Service, Hospital da Beneficência Portuguesa de São Paulo, São Paulo, Brazil 3Infection Control Service, Hospital Israelita Albert Einstein, São Paulo, Brazil 4Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Gent, Belgium

Correspondence Vanda D. Magalhães vanda{at}einstein.br

Received April 15, 2004
Accepted July 2, 2004

An outbreak of bacteraemia in a haemodialysis unit where 65 episodes of infection involved 35 outpatients is reported. Burkholderia cepacia complex was the agent most frequently recovered from blood. Thirty-three environmental and clinical isolates of B. cepacia complex were characterized by whole-cell protein electrophoresis and recA-RFLP profile. Fourteen isolates were genomovar I and 16 isolates were not classifiable by their recA-RFLP pattern. Ribotyping, random amplification of polymorphic DNA (RAPD) and integron profile were used to explore the clonality of the isolates, and revealed multiple strain genotypes. Four ribotypes and RAPD types and three integron patterns were identified. The water supply was identified as the source of the outbreak, and inappropriate cleaning and a leak in the reverse osmosis tubing connection were the probable causes of contamination. B. cepacia complex was still recovered from blood of patients even after apparently adequate measures were taken and water quality standards were met, suggesting that higher standards for water quality should be adopted in haemodialysis units. The genomovars recovered here were distinct from those commonly reported for cystic fibrosis isolates.

Abbreviation: RAPD, random amplification of polymorphic DNA.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
W. Assaad, M. Magalhaes, M. Plesa, C. A. Hart, P. Cornelis, and C. Winstanley
Identical Burkholderia cepacia complex strain types isolated from multiple patients attending a hospital in Brazil
J. Med. Microbiol., February 1, 2006; 55(2): 247 - 249.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2004 Society for General Microbiology.