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J Med Microbiol 53 (2004), 965-974; DOI: 10.1099/jmm.0.45634-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Analysis of gene expression profile in gastric cancer cells stimulated with Helicobacter pylori isogenic strains

Jian-Ping Yuan1, {dagger}, Tao Li1, {dagger}, Hua-Biao Chen2, Zhen-Hong Li1, Gui-Zhen Yang1, Bao-Yu Hu1, Xiao-Dong Shi1, Shan-Qing Tong1, Yi-Xue Li1,3 and Xiao-Kui Guo1

1Department of Medical Microbiology and Parasitology, Shanghai Second Medical University, Shanghai, China 2Institute of Immunology, Second Military Medical University, Shanghai, China 3Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China

Correspondence Xiao-Kui Guo xkguo{at}shsmu.edu.cn

Received February 13, 2004
Accepted June 14, 2004

To understand the biological processes within host cells induced by VacA, isogenic strains of Helicobacter pylori (NCTC 11638 or 11638-{Delta}vacA) were used to stimulate gastric cancer cells SGC7901, and differentially expressed genes in host cells were identified using cDNA microarray technology. More than 300 genes were found to alter their mRNA expression at different time points, among which 68 were related to the cytoskeleton, 87 were associated with cell cycle, cell death and proliferation, IL8 expression was also found to be up-regulated. Cells co-cultured with broth-culture supernatant (BCS) of NCTC 11638 showed more alteration in microtubule cytoskeleton morphology, as observed by laser scanning confocal microscopy, and a lower apoptosis rate, detected by flow cytometry, compared with those co-cultured with BCS of 11638-{Delta}vacA. The supernatants of cells co-cultured with NCTC 11638 showed significantly higher IL8 expression than those co-cultured with 11638-{Delta}vacA. It is concluded that VacA disrupts cytoskeletal architecture by influencing the expression of cytoskeleton-associated genes. VacA breaks the balance between cell proliferation and cell death by inducing the maladjustment of genes related to cell cycle. VacA is also able to induce the inflammatory response.

{dagger}These authors contributed equally to this work.

Abbreviations: BCS, broth-culture supernatant; ROS, reactive oxygen species.




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