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J Med Microbiol 53 (2004), 945-951; DOI: 10.1099/jmm.0.45638-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Sorting a Staphylococcus aureus phage display library against ex vivo biomaterial

Joakim Bjerketorp, Anna Rosander, Martin Nilsson, Karin Jacobsson and Lars Frykberg

Department of Microbiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden

Correspondence Lars Frykberg lars.frykberg{at}mikrob.slu.se

Received February 18, 2004
Accepted June 25, 2004

A phage display library made from Staphylococcus aureus DNA was sorted against a central venous catheter (CVC) that had been removed from a patient 2 days after insertion. After the first panning, approximately 50 % of the clones encoded proteins known to interact with mammalian proteins. After the second and third pannings, fibrinogen-binding and ß2-glycoprotein I (ß2-GPI)-binding phage particles were clearly dominating. Proteins adsorbed to different CVCs were investigated using specific antibodies. Among the proteins probed for, fibrinogen was most abundant, but, interestingly, ß2-GPI was also detected on all tested CVCs.

Abbreviations: CF, clumping factor; CVC, central venous catheter; Fg, fibrinogen; Fn, fibronectin; ß2-GPI, ß2-glycoprotein I.






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