J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dubreuil, J. D.
Right arrow Articles by Del Giudice, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dubreuil, J. D.
Right arrow Articles by Del Giudice, G.
Agricola
Right arrow Articles by Dubreuil, J. D.
Right arrow Articles by Del Giudice, G.
J Med Microbiol 53 (2004), 9-12; DOI: 10.1099/jmm.0.05389-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Effect of heparin binding on Helicobacter pylori resistance to serum

J. Daniel Dubreuil{dagger}, Paolo Ruggiero, Rino Rappuoli and Giuseppe Del Giudice

IRIS Research Center, Chiron Srl, Via Fiorentina 1, 53100 Siena, Italy

Correspondence J. Daniel Dubreuil daniel.dubreuil{at}umontreal.ca

Received July 17, 2003
Accepted September 15, 2003

The objective of this study was to evaluate the effect of heparin binding to Helicobacter pylori cells on their survival in the presence of fresh rabbit serum with or without active complement components. Three H. pylori strains were compared and the amounts of heparin added reflected the physiological concentrations that can be found in animal tissues. No growth of H. pylori was noted in the presence of serum. Serum with or without active complement produced a reduction in c.f.u. for strains SPM 326, CCUG 17874T and SS1. However, addition of heparin resulted in increased survival of bacterial cells in serum with or without active complement. It appears that heparin binding to H. pylori can prevent bacterial cell death due to the alternative complement system. Heparin binding could also protect from heated serum (complement-inactivated), indicating protection from other serum components besides complement. In vivo, the process of heparin binding could possibly result in facilitated colonization due to a higher survival rate.

{dagger}Permanent address: Université de Montréal, Faculté de Médecine Vétérinaire, Dépt de Pathologie et Microbiologie, 3200 rue Sicotte, St-Hyacinthe, Québec, Canada J2S 7C6.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2004 Society for General Microbiology.