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J Med Microbiol 53 (2004), 57-59; DOI: 10.1099/jmm.0.05454-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Preliminary study on rapid identification of Mycobacterium tuberculosis complex isolates by the BD ProbeTec ET system

S. X. Wang, L. H. Sng and L. Tay

Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608

Correspondence S. X. Wang gptwsx{at}sgh.com.sg

Received September 4, 2003
Accepted October 2, 2003

The BD ProbeTec ET system for identification of Mycobacterium tuberculosis complex (MTBC) isolates from BACTEC 12B culture vials was evaluated in comparison with BACTEC NAP (p-nitro-{alpha}-acetylamino-ß-hydroxy-propiophenone) differentiation. Of 145 mycobacterial isolates tested, comprising 89 MTBC and 56 non-tuberculous mycobacteria (NTM), BD ProbeTec ET correctly identified 87 MTBC and 56 NTM but missed two MTBC. Three NTM were misidentified when NAP was incubated at 37 °C only. Overall sensitivity, specificity and positive and negative predictive values were respectively 97.8, 100, 100 and 96.6 % for the BD ProbeTec ET system and 100, 94.6, 96.7 and 94.6 % for NAP.

Abbreviations: AFB, acid-fast bacilli; IAC, internal amplification control; MTBC, Mycobacterium tuberculosis complex; NAP, p-nitro-{alpha}-acetylamino-ß-hydroxy-propiophenone; NTM, non-tuberculous mycobacterium; TB, tuberculosis.




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