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J Med Microbiol 53 (2004), 47-50; DOI: 10.1099/jmm.0.05333-0
© 2004 Society for General Microbiology
ISSN 0022-2615

Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students

Antoinette Ngandjio1,2,3, Maithe Clerc2, Marie Christine Fonkoua1, Jocelyn Thonnon1, Françoise Lunel3, Christiane Bébéar2, Anne Bianchi3,4 and Bertille de Barbeyrac2

1Centre Pasteur du Cameroun, Yaounde, Cameroon 2Laboratoire de Bactériologie, Centre National de Référence des infections à Chlamydia, Université de Bordeaux II, Zone Nord, Bât. 2B, 146 Rue Léo Saignat, 33076 Bordeaux, France 3Institut Alfred Fournier, Paris, France 4Laboratoire Départemental, Conseil Général de Seine Saint Denis, France

Correspondence Bertille de Barbeyrac bertille.de.barbeyrac{at} u-bordeaux2.fr

Received June 3, 2003
Accepted September 22, 2003

Eighteen reference strains of Chlamydia trachomatis were differentiated by omp1 PCR- and nested PCR-based RFLP analysis, using two restriction digestions, one with AluI and the other with the three enzymes HpaII, EcoRI and HinfI. AluI digestion allowed the differentiation of 12 different profiles after CT1/CT5 PCR and 13 different profiles after the nested PCR. The triple hydrolysis permitted the identification of 15 different patterns. In all, 16/18 reference strains were clearly identified. These reference patterns were successfully used to genotype 34 of 35 (28 strains and 7 clinical specimens) samples from infected students, collected during a screening programme in Yaounde (Cameroon). Genotypes D, Da, E, F, G and J were found. The most prevalent omp1 genotype was E (n = 14; 40 %), followed by F (n = 7; 20 %). As RFLP patterns of reference strains are essential for typing clinical isolates, they will greatly facilitate C. trachomatis characterization in many resource-limited laboratories.

Abbreviation: MOMP, major outer-membrane protein.




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M.-C. Hsu, P.-Y. Tsai, K.-T. Chen, L.-H. Li, C.-C. Chiang, J.-J. Tsai, L.-Y. Ke, H.-Y. Chen, and S.-Y. Li
Genotyping of Chlamydia trachomatis from clinical specimens in Taiwan.
J. Med. Microbiol., March 1, 2006; 55(Pt 3): 301 - 308.
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