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J Med Microbiol 52 (2003), 789-791; DOI: 10.1099/jmm.0.05237-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Human rhabdomyosarcoma cells for rapid detection of enteroviruses by shell-vial assay

M. Pérez-Ruiz, J. M. Navarro-Marí, E. Palacios del Valle and M. Rosa-Fraile

Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda Fuerzas Armadas s/n, 18014 Granada, Spain

Correspondence M. Pérez-Ruiz mercedes.perez.ruiz.sspa{at}juntadeandalucia.es

Received February 25, 2003
Accepted June 11, 2003

The ability of the RD (rhabdomyosarcoma) and MRC-5 cell-lines to detect enteroviruses in 33 clinical samples (cerebrospinal fluid, stools and throat swabs) was evaluated. The samples had previously tested enterovirus-positive by traditional tube-culture and had been frozen after their initial processing. By traditional tube-culture, 100 and 85 % of samples were positive for enterovirus in RD and MRC-5 cells, respectively. By rapid shell-vial assay, 94 and 45.5 % were positive after 48 h incubation in RD and MRC-5 cells, respectively. RD cells supported growth of all enterovirus serotypes, whereas MRC-5 cells were not able to detect any of the three coxsackieviruses that were found (one coxsackievirus A9 and two coxsackievirus B5). The shell-vial assay with RD cell-lines may be a useful tool for rapid diagnosis of enteroviral infection.

Abbreviations: CPE, cytopathic effect; CSF, cerebrospinal fluid; IFA, indirect immunofluorescence assay; HEV, human enterovirus; RD, rhabdomyosarcoma; SV, shell-vial assay; TC, tube-culture.




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