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J Med Microbiol 52 (2003), 741-745; DOI: 10.1099/jmm.0.05186-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Anaerobiosis-induced virulence of Salmonella enterica subsp. enterica serovar Typhimurium: role of phospholipase C{gamma} signalling cascade

Madhu Khullar1, Raman Deep Singh1{dagger}, Manu Smriti2 and Nirmal Kumar Ganguly1{ddagger}

Departments of Experimental Medicine and Biotechnology1 and Medical Microbiology2, Postgraduate Institute of Medical Education and Research, Chandigarh 160012, India

Correspondence Madhu Khullar madhu_khullar{at}hotmail.com

Received January 22, 2003
Accepted May 23, 2003

Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) can initiate entry into non-phagocytic epithelial cells by triggering certain signal transduction pathways, thereby allowing the pathogen to invade and establish a niche within host cells. Anaerobiosis has been shown to be an important inducer of the invasion process of S. Typhimurium. However, the effect of anaerobiosis on modulation of cell signalling cascades by S. Typhimurium is not known. In the present study, the phospholipase C{gamma} signalling cascade was investigated in mice enterocytes, following interaction with S. Typhimurium grown under aerobic and anaerobic growth conditions. Significant increases in enterocyte intracellular calcium and inositol 1,4,5-triphosphate levels were observed on interaction with S. Typhimurium grown anaerobically compared with S. Typhimurium grown aerobically. An increased membrane/cytosolic ratio of protein kinase C was also seen with anaerobic S. Typhimurium in enterocytes compared with aerobic S. Typhimurium. These data suggest that anaerobically grown organisms are more efficient in initiating cell-signalling events than are aerobically grown bacteria. These enhanced cell signals may contribute to the increased virulence of S. Typhimurium grown anaerobically.

{dagger}Present address: Mayo Clinic and Foundation, Rochester, MN, USA.

{ddagger}Present address: Director General, ICMR, New Delhi, India.

Abbreviations: IP3, inositol 1,4,5-triphosphate; PKC, protein kinase C; PLC, phospholipase C.




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