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J Med Microbiol 52 (2003), 653-655; DOI: 10.1099/jmm.0.05135-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Faster genetic identification of medically important aspergilli by using gellan gum as gelling agent in mycological media

Dirk Schmidt and Peter-Michael Rath

Institut für Medizinische Mikrobiologie, Universitätsklinikum Essen, Hufelandstrasse 55, D-45122 Essen, Germany

Correspondence Peter-Michael Rath rath{at}uni-essen.de

Received November 27, 2002
Accepted January 3, 2003

Using gellan gum as a substitute for agar-agar in a mycological medium and sequencing of the ITS 1 and 2 regions resulted in an accurate identification of Aspergillus fumigatus, Aspergillus nidulans, Aspergillus terreus and Aspergillus ustus within 24 h of subculture.


Abbreviation: ITS, internal transcribed spacer.




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H. P. Hinrikson, S. F. Hurst, T. J. Lott, D. W. Warnock, and C. J. Morrison
Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species
J. Clin. Microbiol., May 1, 2005; 43(5): 2092 - 2103.
[Abstract] [Full Text] [PDF]




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