| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, CA, USA 2Department of Infectious Diseases, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubiran, Mexico City, Mexico 3State University of New York – Downstate Medical Center, Brooklyn, NY, USA 4Howard Hughes Medical Institute, Albert Einstein College of Medicine, Bronx, NY, USA 5Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA, USA
Correspondence Niaz Banaiee niaz{at}itsa.ucsf.edu
Received December 7, 2002
Accepted February 28, 2003
In a prospective study conducted in a diagnostic laboratory in Mexico City, luciferase reporter mycobacteriophages (LRPs) were evaluated for their utility and performance in identification and antibiotic-susceptibility testing of Mycobacterium tuberculosis complex (MTC) isolates from MGIT-960 cultures. Eighty-four consecutive MGIT cultures recovered from 54 patients were included in this study. The LRPs confirmed mycobacterial growth in 79 (94 %) of 84 MGIT cultures. Failure to confirm growth was due to low inoculum (n = 1) or growth with non-tuberculous mycobacteria (n = 4). The median time to confirmation of MGIT cultures was 1 day (range 1–55). Confirmed cultures were identified with p-nitro-
-acetylamino-ß-hydroxypropiophenone (NAP), a selective inhibitor of MTC species, and results obtained with LRPs were compared with those obtained by BACTEC-460. The sensitivity and specificity of the LRP NAP test were respectively 97 and 100 %, and the median turnaround time for identification was 3 days with both methods. The accuracy and speed of the LRPs for susceptibility testing with rifampicin, streptomycin, isoniazid and ethambutol were compared with BACTEC-460 and discrepant results were tested by the conventional agar proportion method. In total, 72 MTC cultures were tested. The overall agreement between the LRPs and BACTEC-460 was 98.6 %. Four isolates (5.6 %) were falsely identified as ethambutol-resistant. The median turnaround time for susceptibility testing was 3 days (range 3–57) with the LRPs and 9 days (range 7–29) with BACTEC-460. LRPs offer an accurate and rapid approach for identification and susceptibility testing of M. tuberculosis from MGIT-960 cultures.
-acetylamino-ß-hydroxypropiophenone; NTM, non-tuberculous mycobacteria. This article has been cited by other articles:
|
|
 |
|
  N. Parrish, G. Osterhout, K. Dionne, A. Sweeney, N. Kwiatkowski, K. Carroll, K. C. Jost Jr., and J. Dick Rapid, Standardized Method for Determination of Mycobacterium tuberculosis Drug Susceptibility by Use of Mycolic Acid Analysis J. Clin. Microbiol., December 1, 2007; 45(12): 3915 - 3920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rybniker, S. Kramme, and P. L. Small Host range of 14 mycobacteriophages in Mycobacterium ulcerans and seven other mycobacteria including Mycobacterium tuberculosis - application for identification and susceptibility testing J. Med. Microbiol., January 1, 2006; 55(1): 37 - 42. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wiles, K. Ferguson, M. Stefanidou, D. B. Young, and B. D. Robertson Alternative Luciferase for Monitoring Bacterial Cells under Adverse Conditions Appl. Envir. Microbiol., July 1, 2005; 71(7): 3427 - 3432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Pina-Vaz, S Costa-de-Oliveira, and A G Rodrigues Safe susceptibility testing of Mycobacterium tuberculosis by flow cytometry with the fluorescent nucleic acid stain SYTO 16 J. Med. Microbiol., January 1, 2005; 54(1): 77 - 81. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | J MED MICROBIOL | MICROBIOLOGY | J GEN VIROL | ALL SGM JOURNALS |
