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J Med Microbiol 52 (2003), 385-387; DOI: 10.1099/jmm.0.05011-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Improvement of mupirocin E-test for susceptibility testing of Staphylococcus aureus

Pedro Juan José Mondino1,2, Kátia Regina Netto dos Santos1, Maria do Carmo de Freire Bastos1 and Marcia Giambiagi-deMarval1

1Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil 2Departamento de Patologia, Universidade Federal Fluminense, Rio de Janeiro, RJ, Brazil

Correspondence Marcia Giambiagi-deMarval marciagm{at}micro.ufrj.br

Received June 25, 2002
Accepted October 1, 2002

Interpretation of the mupirocin E-test for low-level mupirocin-resistant Staphylococcus aureus strains has been improved by adding the indicator dye tetrazolium. E-tests were compared with agar dilution methods for assessing mupirocin susceptibility. MICs obtained by the agar dilution method and E-tests showed 89.3 % agreement within 2 log2 dilution criteria. The agreement between MICs increased to 100 % in the 1 log2 dilution definition when the indicator dye tetrazolium was added to the E-test. The use of the E-test with tetrazolium reduction is more accurate for determining mupirocin MICs for S. aureus strains.




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N. E. Moreira de Oliveira, A. P. C. M. Cardozo, E. d. A. Marques, K. R. Netto dos Santos, and M. G. deMarval
Interpretive criteria to differentiate low- and high-level mupirocin resistance in Staphylococcus aureus
J. Med. Microbiol., July 1, 2007; 56(7): 937 - 939.
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