HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Samaranayake, Y.H. Articles by Yeung, K.W. S. Search for Related Content PubMed PubMed Citation Articles by Samaranayake, Y.H. Articles by Yeung, K.W. S. Agricola Articles by Samaranayake, Y.H. Articles by Yeung, K.W. S.

`Genotypic shuffling’ of sequential clones of Candida albicans in HIV-infected individuals with and without symptomatic oral candidiasis

¹Oral Bio-sciences, Faculty of Dentistry, University of Hong Kong, Hong Kong ²Department of Biochemistry, Chinese University, Hong Kong

Correspondence L. P. Samaranayake lakshman{at}hkucc.hku.hk

Received 16 May 2002 Accepted 9 December 2002

Although HIV-infected individuals harbour multiple strains of oral Candida albicans, little is known of their micro-evolution over time. Therefore, a prospective study was conducted with 16 HIV-infected ethnic Chinese individuals with and without symptoms of oropharyngeal candidiasis to evaluate the genotype distribution of oral C. albicans isolates during HIV disease progression. Oral-rinse samples were obtained from all individuals and up to five C. albicans colonies were selected for each visit, over a 12 month period of multiple visits. After identification of isolates using standard mycological criteria, the genetic similarities of yeast isolates within and between sequential clones of C. albicans were assessed by DNA fingerprinting through random amplification of polymorphic DNA (RAPD). The results of RAPD gel profiles and the lineage of each isolate were further analysed using commercially available software. RAPD studies revealed the prevalence of up to 14 different genotypes per individual during the study period, with multiple genotypes isolated simultaneously from a single oral rinse. Computer analysis of RAPD profiles revealed that yeasts isolated over sequential visits from symptomatic individuals demonstrated a striking level of relatedness compared with isolates from asymptomatic individuals. Genetically identical C. albicans strains also formed ‘loosely’ connected subclusters that overlapped multiple visits, implying genetic ‘shuffling’ in these isolates during disease progression. These data point to varying evolutionary genetic trends in C. albicans associated with symptomatic oral candidiasis and asymptomatic carriage in HIV disease.

This article has been cited by other articles:

				F. C. Odds, A. D. Davidson, M. D. Jacobsen, A. Tavanti, J. A. Whyte, C. C. Kibbler, D. H. Ellis, M. C. J. Maiden, D. J. Shaw, and N. A. R. Gow Candida albicans Strain Maintenance, Replacement, and Microvariation Demonstrated by Multilocus Sequence Typing. J. Clin. Microbiol., October 1, 2006; 44(10): 3647 - 3658. [Abstract] [Full Text] [PDF]

				K.-W. Chen, Y.-C. Chen, H.-J. Lo, F. C. Odds, T.-H. Wang, C.-Y. Lin, and S.-Y. Li Multilocus Sequence Typing for Analyses of Clonality of Candida albicans Strains in Taiwan. J. Clin. Microbiol., June 1, 2006; 44(6): 2172 - 2178. [Abstract] [Full Text] [PDF]

				M.M. Coogan, P.L. Fidel Jr., M.C. Komesu, N. Maeda, and L.P. Samaranayake (B1) Candida and Mycotic Infections Adv. Dent. Res., April 1, 2006; 19(1): 130 - 138. [Abstract] [Full Text] [PDF]

				K.-W. Chen, H.-J. Lo, Y.-H. Lin, and S.-Y. Li Comparison of four molecular typing methods to assess genetic relatedness of Candida albicans clinical isolates in Taiwan J. Med. Microbiol., March 1, 2005; 54(3): 249 - 258. [Abstract] [Full Text] [PDF]

				Y. Jin, H. K. Yip, Y. H. Samaranayake, J. Y. Yau, and L. P. Samaranayake Biofilm-Forming Ability of Candida albicans Is Unlikely To Contribute to High Levels of Oral Yeast Carriage in Cases of Human Immunodeficiency Virus Infection J. Clin. Microbiol., July 1, 2003; 41(7): 2961 - 2967. [Abstract] [Full Text] [PDF]