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J Med Microbiol 52 (2003), 331-335; DOI: 10.1099/jmm.0.05091-0
© 2003 Society for General Microbiology
ISSN 0022-2615

DIAGNOSTICS, TYPING AND IDENTIFICATION

Evaluation of a rapid bacteriophage-based method for the detection of Mycobacterium tuberculosis in clinical samples

Ayman Mohamed Marei{dagger},1, Eman Mohamed El-Behedy1, Heba Ali Mohtady1 and Afify Fahmy Afify2

Departments of Microbiology and Immunology1 and Internal Medicine2, Zagazig Faculty of Medicine, Egypt

Correspondence Ayman Mohamed Marei am177{at}le.ac.uk


Received 4 October 2002 Accepted 23 December 2002

Rapid, sensitive and low-cost methods are needed urgently for the detection of Mycobacterium tuberculosis in clinical samples, especially in developing countries. To this end, the clinical performance of FASTPlaqueTBTM (a bacteriophage-based method) has been studied in parallel with microscopy, standard microbiological culture and in-house IS6110-based PCR methods. A total of 64 samples, including 42 sputum samples and 22 urine samples, were tested in this study. The sensitivity, specificity and overall accuracy values for the FASTPlaqueTB assay relative to that of culture were respectively 76.5, 95 and 90 %. The corresponding values for the in-house IS6110-based PCR assay were 88, 91 and 90 % and, for Ziehl–Neelsen staining, were 59, 95 and 85 %. FASTPlaqueTB gave better clinical performance with urine samples than with sputum samples (sensitivity, specificity and overall accuracy were 100 % with urine samples and 64, 93 and 84 % with sputum samples). The 100 % sensitivity of FASTPlaqueTB was higher than that of the corresponding values for PCR (67 %) with urine samples. In conclusion, FASTPlaqueTB proved to be sensitive, cheap relative to the PCR and rapid. It is able to detect M. tuberculosis in clinical samples within 1 day, reducing the time to diagnosis in comparison with culture.

{dagger}Present address: Microbiology and Immunology Department, Leicester University, Leicester LE1 9HN, UK.

Abbreviations: LJ, Löwenstein–Jensen; TB, tuberculosis; ZN, Ziehl–Neelsen.




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