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J Med Microbiol 52 (2003), 223-228; DOI: 10.1099/jmm.0.04998-0
© 2003 Society for General Microbiology
ISSN 0022-2615

HOST RESPONSE

Enhancement of DNA vaccine potency against herpes simplex virus 1 by co-administration of an interleukin-18 expression plasmid as a genetic adjuvant

Mingzhao Zhu1, Xuemei Xu1, Hongwei Liu2, Xiaojuan Liu1, Sheng Wang3, Fangtian Dong3, Baoling Yang2 and Guoxing Song1

1,3Department of Biophysics, Institute of Basic Medical Sciences1 and Department of Ophthalmology, Peking Union Hospital3, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, PR China 2Department of Pharmacology, Beijing Institute of Ophthalmology, Beijing 100062, PR China

Correspondence Guoxing Song songgx{at}ms.imicams.ac.cn

Received 13 June 2002 Accepted 15 November 2002

In this study, the immune-modulatory and vaccine effects of using an interleukin (IL)-18 expression plasmid as a genetic adjuvant to enhance DNA vaccine-induced immune responses were investigated in a mouse herpes simplex virus 1 (HSV-1) challenge model. BALB/c mice were immunized by three intramuscular inoculations of HSV-1 glycoprotein D (gD) DNA vaccine alone or in combination with a plasmid expressing mature IL-18 peptide. Both the serum IgG2a/IgG1 ratio and T helper 1-type (Th1) cytokines [IL-2 and interferon (IFN)-{gamma}] were increased significantly by the co-injection of the IL-18 plasmid compared with the injection of gD DNA alone. However, the production of IL-10 was inhibited by IL-18 plasmid co-injection. Furthermore, IL-18 plasmid co-injection efficiently enhanced antigen-specific lymphocyte proliferation and the delayed-type hypersensitivity response. When mice were challenged with HSV-1 at the cornea, co-injection of IL-18 plasmid with gD DNA vaccine showed significantly better protection, manifested as lower corneal lesion scores and faster recovery. These experiments indicate that co-injection of an IL-18 plasmid with gD DNA vaccine efficiently induces Th1-dominant immune responses and improves the protective effect against HSV-1 infection.




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[Abstract] [Full Text] [PDF]


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