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Identification of immunodominant Helicobacter pylori proteins with reactivity to H. pylori-specific egg-yolk immunoglobulin

^1–3Research Center for Gastroenterology¹ and Departments of Gastroenterology² and Surgery³, Dankook University College of Medicine, Cheonan, Korea ⁴Department of Biochemistry, Yonsei University College of Sciences, Seoul, Korea ⁵Department of Agricultural Chemistry, Korea University College of Life & Environmental Science, Seoul, Korea

Correspondence Im-Hwan Roe durig{at}hanmail.net

Received 29 May 2002 Accepted7 November 2002

The importance of hens eggs as a source of specific antibodies (IgY) is well recognized. The protective effect of IgY obtained from hens immunized with Helicobacter pylori whole-cell lysate has been reported for the control of H. pylori infection. However, IgY produced by whole-cell lysates presents the possibility of cross-reactivity with other bacteria, including the normal human flora, and this could decrease the efficiency of IgY. In the present study, the immunodominant proteins of H. pylori with reactivity to H. pylori-specific IgY (IgY-Hp) were identified. IgY obtained from hens immunized with various fractions of H. pylori proteins was isolated and purified, titres of IgY-Hp against H. pylori were determined and cross-reactivity between IgY-Hp and normal human bacteria was examined by Western blot analysis. Finally, immunodominant H. pylori proteins were identified by LC/MS analysis. IgY obtained 2 months after immunization with H. pylori whole-cell lysate showed the highest antibody titre. Five immunodominant proteins were identified that were strongly reactive to IgY-Hp: urease ß-subunit (62 kDa), heat-shock protein 60 (60 kDa), urease -subunit (26 kDa), probable peroxiredoxin (22 kDa) and probable thiol peroxidase (18 kDa). Immunization of hens with the immunodominant proteins identified would produce a more specific IgY against H. pylori.

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