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J Med Microbiol 52 (2003), 151-154; DOI: 10.1099/jmm.0.05012-0
© 2003 Society for General Microbiology
ISSN 0022-2615

DIAGNOSTICS, TYPING AND IDENTIFICATION

Independent subsets of amplified fragments from the genome of Neisseria meningitidis identify the same invasive clones of ET37 and ET5

Jonathan N. Goulding1, John Stanley2, Will Olver3, Keith R. Neal4, Dlawer A. A. Ala'Aldeen3 and Catherine Arnold2

1Department of Academic Paediatrics, Imperial College at St Mary's Hospital, London, UK 2Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK 3,4Molecular Bacteriology and Immunology Group, Division of Microbiology3 and Department of Public Health Medicine and Epidemiology4, University of Nottingham, Queen's Medical Centre, Nottingham, UK

Correspondence Catherine Arnold carnold{at}phls.org.uk

Received 25 June 2002 Accepted 14 October 2002

To determine whether fluorescent amplified-fragment length polymorphism (FAFLP) analysis is an unbiased genome sampling technique, data were analysed from three different primer combinations, amplifying three independent fragment subsets from 123 isolates of Neisseria meningitidis. Using these data, dendrograms were generated with near-identical topologies that identified the same invasive clones of ET37 and ET5 and also identified the same outbreak clusters.






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