HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplementary table Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Woo, P. C.-Y. Articles by Yuen, K.-Y. Search for Related Content PubMed PubMed Citation Articles by Woo, P. C.-Y. Articles by Yuen, K.-Y. Agricola Articles by Woo, P. C.-Y. Articles by Yuen, K.-Y.

Granulicatella adiacens and Abiotrophia defectiva bacteraemia characterized by 16S rRNA gene sequencing

¹Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong ²Department of Microbiology, Tuen Mun Hospital, Hong Kong ³Department of Microbiology, Pamela Youde Nethersole Eastern Hospital, Hong Kong ⁴HKU–Pasteur Research Centre, Hong Kong

Correspondence Kwok-Yung Yuen hkumicro{at}hkucc.hku.hk

Received 19 April 2002 Accepted17 October 2002

Traditionally, the identification, epidemiology and spectrum of clinical diseases caused by Granulicatella adiacens and Abiotrophia defectiva are dependent upon their phenotypic characterization. During a 6-year period (July 1995–June 2001), seven and two -haemolytic streptococci were identified as G. adiacens and A. defectiva, respectively, by 16S rRNA gene sequencing. Three patients with haematological malignancies and neutropenic fever had primary bacteraemia. Three patients with valvular problems or congenital heart disease had infective endocarditis. A patient with ischemic heart disease and cerebrovascular accident had infected aortic atheroma with dissection. A patient with recurrent pyogenic cholangitis had acute cholangitis and a patient with polypoid cystitis and benign prostatic hypertrophy had acute prostatitis. Four of the nine patients died, including all three with G. adiacens infective endocarditis or infected atheroma. For the seven G. adiacens isolates, the API 20 STREP system successfully identified one and five isolates as G. adiacens with >95 % and 80–90 % confidence, respectively, whereas the Vitek System (GPI) and ATB Expression system (ID32 STREP) successfully identified none and one isolate as G. adiacens. Of the two A. defectiva isolates, none of the three systems successfully identified either of them as A. defectiva. 16S rRNA gene sequencing is the technique of choice for identifying G. adiacens and A. defectiva, and early surgical intervention should be considered when G. adiacens endocarditis is diagnosed.

This article has been cited by other articles:

				S K P Lau, P C Y Woo, N K H Li, J L L Teng, K-W Leung, K H L Ng, T-L Que, and K-Y Yuen Globicatella bacteraemia identified by 16S ribosomal RNA gene sequencing. J. Clin. Pathol., March 1, 2006; 59(3): 303 - 307. [Abstract] [Full Text] [PDF]

				P. C. Y. Woo, H. Tse, S. S. Y. Wong, C. W. S. Tse, A. M. Y. Fung, D. M. W. Tam, S. K. P. Lau, and K.-y. Yuen Life-Threatening Invasive Helcococcus kunzii Infections in Intravenous-Drug Users and ermA-Mediated Erythromycin Resistance J. Clin. Microbiol., December 1, 2005; 43(12): 6205 - 6208. [Abstract] [Full Text] [PDF]

				J. A. Aas, B. J. Paster, L. N. Stokes, I. Olsen, and F. E. Dewhirst Defining the Normal Bacterial Flora of the Oral Cavity J. Clin. Microbiol., November 1, 2005; 43(11): 5721 - 5732. [Abstract] [Full Text] [PDF]

				X. Zheng, A. F. Freeman, J. Villafranca, D. Shortridge, J. Beyer, W. Kabat, K. Dembkowski, and S. T. Shulman Antimicrobial Susceptibilities of Invasive Pediatric Abiotrophia and Granulicatella Isolates J. Clin. Microbiol., September 1, 2004; 42(9): 4323 - 4326. [Abstract] [Full Text] [PDF]

				P. C. Y. Woo, A. P. C. To, S. K. P. Lau, A. M. Y. Fung, and K.-y. Yuen Phenotypic and Molecular Characterization of Erythromycin Resistance in Four Isolates of Streptococcus-Like Gram-Positive Cocci Causing Bacteremia J. Clin. Microbiol., July 1, 2004; 42(7): 3303 - 3305. [Abstract] [Full Text] [PDF]

				P C Y Woo, S K P Lau, A M Y Fung, S K Chiu, R W H Yung, and K Y Yuen Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing J. Clin. Pathol., September 1, 2003; 56(9): 690 - 693. [Abstract] [Full Text] [PDF]

				P. C. Y. Woo, K. H. L. Ng, S. K. P. Lau, K.-t. Yip, A. M. Y. Fung, K.-w. Leung, D. M. W. Tam, T.-l. Que, and K.-y. Yuen Usefulness of the MicroSeq 500 16S Ribosomal DNA-Based Bacterial Identification System for Identification of Clinically Significant Bacterial Isolates with Ambiguous Biochemical Profiles J. Clin. Microbiol., May 1, 2003; 41(5): 1996 - 2001. [Abstract] [Full Text] [PDF]