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J Med Microbiol 52 (2003), 121-125; DOI: 10.1099/jmm.0.05017-0
© 2003 Society for General Microbiology
ISSN 0022-2615

HOST RESPONSE

Immune response to native NadA from Neisseria meningitidis and its expression in clinical isolates in Brazil

Lucila O. Fukasawa12, Maria Cecília O. Gorla3, Ana Paula S. Lemos3, Rocilda P. F. Schenkman1, Maria Cristina C. Brandileone3, Jay W. Fox4, Isaias Raw1, Carl E. Frasch5 and Martha M. Tanizaki1

1Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brasil 1500, CEP 05504-900, São Paulo, Brazil 2Curso de Pós-Graduação em Biotecnologia, USP-Butantan-IPT, Brazil 3Serviço de Bacteriologia, Instituto Adolfo Lutz, São Paulo, Brazil 4Department of Microbiology, University of Virginia, Charlottesville, VA, USA 5Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, USA

Correspondence Martha M. Tanizaki tanizaki{at}usp.br

Received 17 July 2002 Accepted 3 October 2002

A mAb against the NadA protein from Neisseria meningitidis strain 3006 (serosubtype B : 2b : P1.2 : P5.2,8) demonstrated strong bactericidal activity against Brazilian epidemic serogroup B strain N44/89 (B : 4,7 : P1.19,15 : P5.5,7) and a serogroup C strain, IMC 2135 (C : 2a : P1.5,2), but not against another serogroup C strain, N1002/90 (C : 2b : P1.3 : P5.8). The immunogenicity of native NadA in an outer-membrane vesicle (OMV) preparation was also tested. Serum from mice immunized with OMV from serogroup B strain N44/89, which contains the NadA protein, showed bactericidal activity against serogroup B and C strains possessing NadA. In dot-blot analysis of 100 serogroup B and 100 serogroup C isolates from Brazilian patients, the mAb to NadA recognized about 60 % of the samples from both serogroups. The molecular mass of the NadA protein from strain N44/89 determined by mass spectrometry was 37 971 Da and the peptide sequences were identical to those of NadA from N. meningitidis strain MC58.




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