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J Med Microbiol 52 (2003), 1077-1081; DOI: 10.1099/jmm.0.05371-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Description of new mutations in the rpoB gene in rifampicin-resistant Neisseria meningitidis selected in vitro in a stepwise manner

Oliver Nolte1, Matthias Müller1, Stephan Reitz1, Sandra Ledig1, Ingrid Ehrhard2 and Hans-Günther Sonntag1

1Hygiene Institute, Dept of Hygiene and Medical Microbiology, University of Heidelberg, Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany 2Landesuntersuchungsanstalt für das Gesundheits- und Veterinärwesen (LUA) Sachsen, Standort Dresden, Abt. Med. Mikrobiologie und Hygiene, Haus Jägerstr. 10, D-01099 Dresden, Germany

Correspondence Oliver Nolte Oliver_Nolte{at}med. uni-heidelberg.de

Received July 1, 2003
Accepted August 19, 2003

Fourteen meningococcal strains were selected towards rifampicin resistance in a stepwise manner in vitro; final MICs were between 8 and >256 µg ml-1. Sequence analysis of a 295 bp subgenic fragment of the RNA polymerase ß-subunit (rpoB) gene from the original and the fully resistant strains revealed that, with one exception, the strain pairs differed by just one position in the deduced amino acid sequence. Transformation of a PCR-amplified subgenic rpoB fragment harbouring the mutated site into a susceptible strain demonstrated the resistance-conferring mechanism.




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