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PFGE and pertactin gene sequencing suggest limited genetic variability within the Finnish Bordetella parapertussis population

^1,2National Public Health Institute, Department of Human Microbial Ecology and Inflammation¹ and Turku Graduate School of Biomedical Sciences, University of Turku², Turku, Finland ³Department of Pediatrics, Turku University Hospital, Turku, Finland ⁴Department of Medical Microbiology, University of Turku, Turku, Finland ⁵Unité des Bordetella, Centre National de Référence des Bordetelles, Institut Pasteur, Paris, France

Correspondence Johanna Mäkinen johanna.makinen{at}utu.fi

Received August 18, 2003
Accepted August 28, 2003

The outer-membrane protein pertactin (Prn) of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica is believed to function as an adhesin and is an important immunogen. The emergence of B. pertussis and B. bronchiseptica Prn variants has been reported. The aim of this study was to determine whether similar variation is found in B. parapertussis Prn and to characterize Finnish clinical B. parapertussis isolates that were collected in 1982–2000. Of 76 B. parapertussis isolates studied, seven (9 %) were found to have silent and non-silent nucleotide changes. In addition, one (1 %) had eight PQP repeats instead of nine. Three closely related B. parapertussis XbaI PFGE patterns were found. Genetic variation of B. parapertussis was found to be very limited, suggesting that B. parapertussis is a stable organism that is well-adapted to its own ecological niche.

The GenBank/EMBL/DDBJ accession numbers for the sequences of polymorphic regions 1 and 2 of Bordetella parapertussis pertactin are AF503929 and AF503928, respectively.

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