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J Med Microbiol 52 (2003), 1021-1026; DOI: 10.1099/jmm.0.05234-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Experimental Ehrlichia chaffeensis infection in beagles

Xiao-Feng Zhang1, Jian-Zhi Zhang1, S. Wesley Long1, Randall P. Ruble2 and Xue-Jie Yu1

Departments of Pathology and Microbiology and Immunology, Center for Biodefense and Emerging Infectious Diseases1 and Animal Resource Center2, University of Texas Medical Branch, Galveston, Texas 77555-0609, USA

Correspondence Xue-Jie Yu xuyu{at}utmb.edu

Received February 25, 2003
Accepted August 8, 2003

A canine model for human monocytic ehrlichiosis was used to assess persistent infection and antigenic variation of Ehrlichia chaffeensis. Two beagle dogs were infected subcutaneously with E. chaffeensis Arkansas strain. The dogs were observed for 6 months after inoculation for clinical signs, blood chemistry changes, antibodies to E. chaffeensis and presence of E. chaffeensis in the blood. Both dogs developed thrombocytopenia, but exhibited normal body temperatures during the entire course of infection. In one dog, E. chaffeensis was cultivated for up to 74 days post-inoculation and E. chaffeensis DNA was detected in the dog's blood for up to 81 days. In the other dog, E. chaffeensis was cultured for up to 102 days and E. chaffeensis DNA was detected in the blood for up to 117 days. PCR amplification and DNA sequence analysis indicated that there was no genetic variation in the 120 kDa outer-membrane glycoprotein gene of E. chaffeensis during infection of the dogs. The dogs developed antibodies to the immunodominant proteins of E. chaffeensis, including the 175, 140, 120, 80, 50 and 28 kDa proteins, starting in the fifth week post-inoculation. The dogs maintained high antibody titres throughout the 6-month study period. These results indicate that dogs become carriers of E. chaffeensis for 2–4 months after infection without exhibiting signs of clinical disease, suggesting that dogs may serve as a natural host for E. chaffeensis.

Abbreviations: IFA, immunofluorescence assay; UTMB, University of Texas Medical Branch.




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