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J Med Microbiol 52 (2003), 893-896; DOI: 10.1099/jmm.0.05290-0
© 2003 Society for General Microbiology
ISSN 0022-2615

Real-time PCR to improve the diagnosis of respiratory syncytial virus infection

R. Mentel1, U. Wegner1, R. Bruns2 and L. Gürtler1

Friedrich Loeffler Institute of Medical Microbiology1 and Department of Paediatrics2, University Hospital, Ernst-Moritz-Arndt University Greifswald, Germany

Correspondence Renate Mentel Renate.Mentel{at}uni- greifswald.de

Received April 23, 2003
Accepted July 17, 2003

Respiratory syncytial virus (RSV) is one of the most important virus respiratory pathogens in infants and young children. A rapid and sensitive diagnosis is essential to focus any outbreak due to this virus. A real-time RT-PCR method was designed using a primer/probe pair from the F gene. Simultaneously with nested RT-PCR and antigen ELISA, 71 consecutive specimens from hospitalized children with clinical symptoms of acute respiratory distress were evaluated to confirm the incidence of RSV infection. RSV was detected in 25 (35.2 %) specimens by real-time RT-PCR and in 19 (26.7 %) by nested RT-PCR. The assay was specific for RSV. The procedure offers a rapid and sensitive alternative to conventional RT-PCR. Closed-tube detection eliminates the risk of contamination.

Abbreviation: RSV, respiratory syncytial virus.




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