J Med Microbiol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Héchard, C.
Right arrow Articles by Rodolakis, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Héchard, C.
Right arrow Articles by Rodolakis, A.
Agricola
Right arrow Articles by Héchard, C.
Right arrow Articles by Rodolakis, A.
J Med Microbiol 52 (2003), 35-40; DOI: 10.1099/jmm.0.04983-0
© 2003 Society for General Microbiology
ISSN 0022-2615

HOST RESPONSE

Evaluation of protection against Chlamydophila abortus challenge after DNA immunization with the major outer-membrane protein-encoding gene in pregnant and non-pregnant mice

Céline Héchard{dagger}, Olivier Grépinet and Annie Rodolakis

Unité de Pathologie Infectieuse et Immunologie, INRA – Centre de Tours, 37380 Nouzilly, France

Correspondence Olivier Grépinet grepinet{at}tours.inra.fr

Received 5 June 2002 Accepted 21 August 2002

The protective effect of DNA vaccination with the gene encoding the major outer-membrane protein (MOMP) of Chlamydophila abortus has been studied in non-pregnant and pregnant mouse models after chlamydial challenge. OF1 outbred mice were vaccinated intramuscularly three times every 3 weeks, mated and challenged with C. abortus 2 weeks after the last injection of DNA. In non-pregnant mice, the MOMP DNA vaccine elicited a specific humoral response with predominantly IgG2a antibodies, suggesting a Th1-type immune response. The induced antibodies showed no in vitro neutralizing effect on C. abortus infectivity. Moreover, immunization with the momp gene showed no reduction in the mean splenic bacterial counts of non-pregnant or pregnant mice or in the mean placental bacterial counts of pregnant mice after the C. abortus challenge. Nevertheless, the MOMP DNA immunization induced a non-specific and partial protection in fetuses against challenge.




This article has been cited by other articles:


Home page
 J Med MicrobiolHome page
C. Hechard, O. Grepinet, and A. Rodolakis
Molecular cloning of the Chlamydophila abortus groEL gene and evaluation of its protective efficacy in a murine model by genetic vaccination
J. Med. Microbiol., September 1, 2004; 53(9): 861 - 868.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL J MED MICROBIOL MICROBIOLOGY J GEN VIROL ALL SGM JOURNALS
Copyright © 2003 Society for General Microbiology.