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J. Med. Microbiol. -- Vol. 51 (2002), 710-712
© 2002 Society for General Microbiology
ISSN 0022-2615

MOLECULAR DIAGNOSTICS

Early identification of Mycobacterium tuberculosis complex in BACTEC cultures by ligase chain reaction

S.X. WANG and L. TAY

Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Republic of Singapore

Corresponding author: Dr Tay Leng (e-mail: gpttay{at}sgh.com.sg).

Received 20 Sept. 2001; revised version accepted 4 April 2002.

Abstract

A total of 1431 acid-fast bacilli (AFB) in Bactec® culture vials from 1427 patients was differentiated by the Bactec NAP® method and tested by the LCx Mycobacterium tuberculosis ligase chain reaction system. In all, 1321 of 1325 M. tuberculosis complex (MTBC) isolates were correctly detected by the LCx assay. All the 106 non-tuberculous mycobacteria (NTM) isolates were negative by the LCx assay. No false MTBC-positive result was obtained from NTM isolates. However, the LCx assay failed to detect four MTBC isolates from one patient. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were 99.7, 100%, 100% and 96.4%, respectively. These data suggest that the LCx system can be used to identify MTBC in AFB-positive Bactec broth cultures when the growth index is >=100. The method gives a 100% PPV and allows a faster turnaround time for MTBC than the NAP test.




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