HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by SOSROSENO, W. Articles by HERMINAJENG, E. Search for Related Content PubMed PubMed Citation Articles by SOSROSENO, W. Articles by HERMINAJENG, E. Agricola Articles by SOSROSENO, W. Articles by HERMINAJENG, E.

The role of macrophages in the induction of murine immune response to Actinobacillus actinomycetemcomitans

WIHASKORO SOSROSENO^*, and ENDANG HERMINAJENG

*Department of Oral Biology, School of Dental Science, Universiti Sains Malaysia, 16150 Kota Bharu, Kelantan, Malaysia and Departments of Oral Biology and Oral Medicine, Faculty of Dentistry, Gadjah Mada University, Yogyakarta 55281, Indonesia

Corresponding author: Dr W. Sosroseno (e-mail: wihaskoro{at}kb.usm.my).

Received 31 May 2001; revised version received 11 November 2001; accepted 2 January 2002.

Abstract

The aim of this study was to determine the role of macrophages in the Actinobacillus actinomycetemcomitans-induced murine immune response. BALB/c mice were given carrageenan solution by intraperitoneal injection before immunisation with heat-killed A. actinomycetemcomitans. Mice immunised with antigens and phosphate-buffered saline served as positive and negative controls, respectively. One week after the last immunisation, the delayed-type hypersensitivity (DTH) response was assessed by measurement of footpad swelling. Serum IgG and IgM anti-A. actinomycetemcomitans antibody levels and culture supernate levels of interferon (IFN)- were determined by ELISA. The diameter of abscess formation was determined every 5 days. Sham-immunised spleen cells were transferred to carrageenan-untreated recipients (groups A and B) and to carrageenan-treated recipients (group D). Antigen-immunised spleen cells were transferred to carrageenan-untreated (group C) and carrageenan-treated (group E) recipients. The carrageenan-treated recipients in groups F and G received macrophages from antigen- and sham-immunised mice respectively. All mice except those in group A were immunised with antigen 24 h after cell transfer. After 1 week, a partial suppression of DTH response, reduced levels of IFN-, serum IgG and IgM anti-A. actinomycetemcomitans antibodies and delayed healing were seen in carrageenan-treated mice when compared with the positive control. The immune response to A. actinomycetemcomitans in groups A, B and D was lower than that in groups C and E. Healing of the lesion in the former groups was also delayed when compared with the latter groups. The immune response and the healing of the lesion could be partially restored in carrageenan-treated mice that received antigen-pulsed macrophages (group F) but not in those that received naïve macrophages (group G). These results suggest that macrophages play a partial role in the induction of the murine immune response to A. actinomycetemcomitans.

This article has been cited by other articles:

				H. A. Phelps and M. N. Neely SalY of the Streptococcus pyogenes Lantibiotic Locus Is Required for Full Virulence and Intracellular Survival in Macrophages Infect. Immun., September 1, 2007; 75(9): 4541 - 4551. [Abstract] [Full Text] [PDF]

				O. Goldmann, M. Rohde, G. S. Chhatwal, and E. Medina Role of Macrophages in Host Resistance to Group A Streptococci Infect. Immun., May 1, 2004; 72(5): 2956 - 2963. [Abstract] [Full Text] [PDF]