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J. Med. Microbiol. -- Vol. 51 (2002), 417-422
© 2002 Society for General Microbiology
ISSN 0022-2615

MOLECULAR DIAGNOSTICS

DNA-PCR and RT-PCR for the 18-kDa gene of Mycobacterium leprae to assess the efficacy of multi-drug therapy for leprosy

GUE-TAE CHAE, MIN-JOO KIM, TAE-JIN KANG, SEONG-BEOM LEE, HANG-KYE SHIN*, JONG-PILL KIM*, YOUNG-HOON KO*, SUNG-HWA KIM{dagger} and NAN-HEE KIM{ddagger}

Institute of Hansen's Disease, Department of Pathology, Catholic University of Korea, Seoul 137-701, *Affiliated Hospital, Korean Leprosy Control Association, Euiwang City, Kyunggi-Do 437-823, {dagger}Catholic Skin Clinic, Taegu City 702-200 and {ddagger}Jesus Hospital, Leprosy Mission, Taegu City 704-080, Korea

Corresponding author: Dr G.-T. Chae (e-mail guetae{at}cmc.cuk.ac.kr).

Received 7 June 2001; revised version received 27 Oct.; accepted 5 Dec. 2001.

Abstract

DNA-PCR and reverse transcription (RT)-PCR for the 18-kDa protein of Mycobacterium leprae were used to examine the efficacy of multi-drug therapy (MDT) in leprosy. MDT was administered for 0–24 months. Fourteen (63.6%) of 22 patients showed positive PCR results after treatment for 12 months and the positive results decreased to 30% after 24 months of MDT. These results did not correlate with the bacterial index (BI) or the IgM antibody titre for the phenolic glycolipid (PGL)-1. One-dimensional densitometric analysis of agarose gels from PCR from the longitudinal study showed a gradual reduction of the 360-bp band after 12–24 months of MDT. RT-PCR for mRNA of the 18-kDa protein successfully tracked bacterial RNA changes in the biopsies and confirmed a decrease in the RNA of M. leprae in patients after MDT for 12 months. Thus, DNA- and RT-PCR for the 18-kDa protein of M. leprae are effective in assessing the efficacy of MDT for leprosy.




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