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Clonal structure of Shiga toxin (Stx)-producing and ß-D-glucuronidase-positive Escherichia coli O157:H7 strains isolated from outbreaks and sporadic cases in Hokkaido, Japan

Hokkaido Institute of Public Health, North-19, West-12, Kita-ku, Sapporo 060-0819, Japan

Corresponding author: Dr H. Nagano (e-mail: nagano{at}iph.pref.hokkaido.jp).

Received 15 Oct. 2001; accepted 28 Nov. 2001.

Abstract

A total of 22 clonal phenotypic variants of Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 was isolated from six different locations in Hokkaido, Japan. These isolates were negative for sorbitol fermentation but positive for ß-D-glucuronidase (GUD⁺). They carried eaeA, EHEC-hlyA, pas and etpD genes like typical E. coli O157:H7 and, in addition, stx1 and stx2 genes. However, they were shown to lack katP and espP genes that are present in typical STEC O157:H7. All these atypical GUD⁺ STEC O157:H7 isolates had very similar antimicrobial susceptibilities. Pulsed-field gel electrophoresis analysis with XbaI, SfiI, SwaI, SpeI and NotI indicated that they were identical or closely related to one another. From their phenotypic and genotypic features, these GUD⁺ STEC O157:H7 isolates may represent a distinct clone among STEC O157.

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