HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by KOBAYASHI, D. Articles by KOIKE, M. Search for Related Content PubMed PubMed Citation Articles by KOBAYASHI, D. Articles by KOIKE, M. Agricola Articles by KOBAYASHI, D. Articles by KOIKE, M.

Gastric mucosal density of Helicobacter pylori estimated by real-time PCR compared with results of urea breath test and histological grading

Department of Human Pathology, School of Medicine, Tokyo Medical and Dental University, Tokyo and *Department of Gastroenterology, Juntendo University, School of Medicine, Tokyo, Japan

2001. Corresponding author: Dr Y. Eishi (e-mail: eishi.path{at}tmd.ac.jp).

Received 20 Aug. 2001; revised version accepted 8 Oct.

Abstract

The accuracy of the urea breath test (UBT) and histological grading for estimation of the density of Helicobacter pylori in gastric mucosa is not known. Real-time (TaqMan) PCR was used to estimate the total number of H. pylori genomes in biopsy samples. These values were compared with those obtained by the UBT and the histological grade obtained by the Sydney system. The UBT and endoscopy with antral and corporal biopsies were performed in 88 consecutive untreated patients with dyspepsia. Bacterial culture and the rapid urease test were done with fresh biopsy materials. TaqMan PCR and histological examination were done on serial paraffin sections of the biopsy samples. Of the five methods tested, TaqMan PCR had the highest sensitivity and specificity (both 100%) in the diagnosis of H. pylori infection. The mean density of H. pylori genomes for pairs of biopsy samples from individual patients was compared with the individual values obtained by the UBT; correlation between the results was significant. The density of H. pylori genomes was higher in histological grades 1, 2 and 3 than in grade 0, without significant differences between adjacent grades from 1 to 3. These results suggest that the severity of H. pylori infection of the stomach can be estimated by the UBT and that histopathologists might state whether the organism is present or absent, rather than making a quantitative statement as recommended in the Sydney system.

This article has been cited by other articles:

				K. Miki, Y. Urita, F. Ishikawa, T. Iino, H. Shibahara-Sone, R. Akahoshi, S. Mizusawa, A. Nose, D. Nozaki, K. Hirano, et al. Effect of Bifidobacterium bifidum Fermented Milk on Helicobacter pylori and Serum Pepsinogen Levels in Humans J Dairy Sci, June 1, 2007; 90(6): 2630 - 2640. [Abstract] [Full Text] [PDF]

				F. Megraud and P. Lehours Helicobacter pylori Detection and Antimicrobial Susceptibility Testing Clin. Microbiol. Rev., April 1, 2007; 20(2): 280 - 322. [Abstract] [Full Text] [PDF]

				T. H. FARAG, R. J. STOLTZFUS, S. S. KHALFAN, and J. M. TIELSCH HELICOBACTER PYLORI INFECTION IS ASSOCIATED WITH SEVERE ANEMIA OF PREGNANCY ON PEMBA ISLAND, ZANZIBAR Am J Trop Med Hyg, March 1, 2007; 76(3): 541 - 548. [Abstract] [Full Text] [PDF]

				C. Schabereiter-Gurtner, A. M. Hirschl, B. Dragosics, P. Hufnagl, S. Puz, Z. Kovach, M. Rotter, and A. Makristathis Novel Real-Time PCR Assay for Detection of Helicobacter pylori Infection and Simultaneous Clarithromycin Susceptibility Testing of Stool and Biopsy Specimens J. Clin. Microbiol., October 1, 2004; 42(10): 4512 - 4518. [Abstract] [Full Text] [PDF]

				J. W. Fahey, A. Munoz, Y. Matsuzaki, H. Suzuki, P. Talalay, M. Tauchi, S. Zhang, C. Hurt, and A. Yanaka Dietary Amelioration of Helicobacter pylori Infection: Design Criteria for a Clinical Trial Cancer Epidemiol. Biomarkers Prev., October 1, 2004; 13(10): 1610 - 1616. [Abstract] [Full Text] [PDF]

				C. Lascols, D. Lamarque, J.-M. Costa, C. Copie-Bergman, J.-M. Le Glaunec, L. Deforges, C.-J. Soussy, J.-C. Petit, J.-C. Delchier, and J. Tankovic Fast and Accurate Quantitative Detection of Helicobacter pylori and Identification of Clarithromycin Resistance Mutations in H. pylori Isolates from Gastric Biopsy Specimens by Real-Time PCR J. Clin. Microbiol., October 1, 2003; 41(10): 4573 - 4577. [Abstract] [Full Text] [PDF]