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A comparison of the isolation rates of Salmonella and thermophilic Campylobacter species after direct inoculation of media with a dilute faecal suspension and undiluted faecal material

Public Health Laboratory Service (Midlands), Group Headquarters, Royal Shrewsbury Hospital, Mytton Oak Road, Shrewsbury SY3 8XQ and *PHLS Statistics Unit, 61 Colindale Avenue, London NW9 5EQ

Corresponding author: Dr K.J. Nye.

Received 29 Jan. 2000; revised version accepted 29 June 2000.

Abstract

Regardless of media used, dilution of faecal samples before direct plating may improve isolation rates and reduce subcultures by freeing organisms from the faecal mass and diminishing competing flora. Despite the routine use of dilution in many laboratories, it has never been established properly whether direct or dilute inocula should be used in primary plating of faeces. A total of 3764 faecal samples was examined in four laboratories with a standardised methodology. The isolation rates, competing flora and confirmatory work performed for Salmonella spp. and Campylobacter spp. from primary plating media with a dilute faecal inoculum were compared with those after direct inoculation of faecal material. Inoculum effects on the isolation of Shigella spp. could not be assessed as only one isolate occurred during the study period. The overall isolation rates of both major enteric pathogens were unaffected by the inoculum. However, significantly fewer wasted subcultures were recorded with a dilute inoculum for Campylobacter spp., and competing flora was reduced in all cases without diluting out small numbers of the pathogen.

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